Hello everybody,

I have a problem with SDS-PAGE and I do not know why my samples stop at the top of the resolving gel and they do not run through it. The percentage of gel is 15% and the size of the protein is 25 KDa. They are samples after purification with imidazole and I purified these samples and other similar samples with the same protocol and method of preparing the gel and performing SDS-PAGE successfully (I did not change buffers or other things).

Any advice and suggestions will be greatly appreciated.

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