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Questions related from Sara Heidarpanah
Dear friends, Does anyone know how can I re-charge HisTrap HP 5 ml column? I use HisTrap HP 5 ml column to purify proteins and since I used it multiple times I had to re-charge it but it was not...
04 August 2021 7,177 5 View
Hello everybody, I have a problem with SDS-PAGE and I do not know why my samples stop at the top of the resolving gel and they do not run through it. The percentage of gel is 15% and the size of...
15 February 2021 9,359 6 View
Hi everybody, Does anyone have a good protocol for protein purification using AKTA? I have tried to purify two proteins but after several attempts it was not successful. So, I really appreciate...
29 December 2020 5,438 15 View
Hi everybody, I have a problem with protein purification and I hope that I find the answer here. I used pET151/D-TOPO® for cloning and expression of three candidates. All of them have been...
18 November 2020 7,467 12 View
Hello everybody, I have a problem with western blot of my target protein (the size of protein is around 14 KDa). I observed band of target protein in SDS-PAGE of samples after 1,2,3 and 4 hours...
04 December 2019 3,376 3 View
Hello everybody, Does anyone have an experience with direct colony sequencing? Last month, I transformed TOPO® Cloning reaction into TOP10 cells. I analyzed positive transformants using Colony...
17 February 2019 8,877 8 View
