What's a nice starting reference on calculation of off-diagonal spin correlations using the directed loop (SSE) algorithm?

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How to store fish tissue sample for proximate analysis and antioxidant enzyme analysis?

I need to analyse proximate composition in whole fish and antioxidant enzyme analysis. What is the protocol to store these samples for maximum efficiency in the results. I have -80 degree C...

05 June 2024 1,371 6 View

Acute toxicity test is not showing proper results. Want to know what could be the factors?

When we started the experiment at first at 1ppm all the fishes died within 12 hours. repeated the experiments with lower concentrations starting from 0.05 ppm and found a very good response to the...

02 March 2024 3,096 8 View

How to solve Fuzzy Boundary Value Problem by using Fuzzy Laplace Transform Method?

There are so many papers available to solve initial value problem by using Fuzzy Laplace Transform method. But need solution for the boundary value problem by using the same method.

29 November 2023 9,727 2 View

Can interpretations based on non-parametric tests be accepted for non-normally distributed sample in the fields of Experimental/Survey Research?

For a non-normally distributed small sample (N

20 November 2023 1,952 4 View

When i going to do Rietveld Refinement in X-pert highscore plus it is showing negetive intensities in scan data points.. Why it is showing?

Actually I want to insert hkl values for my peaks in XRD pattern.. hkl values are not coming automatically in x-pert highscore plus software. So i have to do Rietveld Refinement. But when i am...

12 December 2022 8,783 8 View

How to achieve denaturation and renaturation of protein from pellet fraction of the cell (BL21 DE3)?

I am struggling with a Mycobacterium protein (phthalate esterase), which upon overexpression forms inclusion body (maybe) and it is found in the pellet fraction. I have tried cloning the protein...

27 July 2022 2,715 6 View

Lost protein (phthalate hydrolase) activity which I could achieve once but in later attempts the protein is not showing function? Please suggest??

I have cloned a phthalate hydrolase (~37 kDa) into a pMAL-c2x vector and overexpressed in E.coli BL21(DE3). Induction condition - 28 degrees for 3h with 0.5mM IPTG. This is a Mycolicibacterium...

07 June 2022 6,485 4 View

How to calculate protein content per gram of tissue sample in Fish Tissue?

I have used Lowry method to calculate protein content in fish muscle. Briefly, 5% tissue homogenate is prepared in 0.25 M sucrose. Centrifuged and the supernatant is used for analysis. 0.1 mL of...

31 May 2022 6,203 0 View

How metal is extracted from a leached solution?

I was going though this paper "Extraction of vanadium from high calcium vanadium slag using direct roasting and soda leaching" but finally how could they extract from the leached solution I didn;t...

02 September 2021 4,166 3 View

Can anybody provide name of supplier of intra-vaginal progesterone sponge for goat/ sheep?

A project of estrus synchronization in sheep and goat has been started. Intra-vaginal commercial progesterone sponge is required.

03 August 2021 9,524 4 View

Why my unstained, isotype (IgG) and positive samples (U87 cells) have same histogram results?

Hi, I just want to ask regarding flow cytometry method for indirect staining of U87 cells (Glioblastoma) as I'm still quite new in using this method. My objective is to observe the expression of...

23 March 2024 8,882 1 View

How do you deal with the right shift of histogram in imaging?

Good Day I am currently reviewing concepts, and practicing using a epifluorescent microscopy. I often notice that there is a right shift in the histogram of my images. I would like to ask/seek...

18 August 2023 3,438 0 View

Interpreting the results of a GAMM model and plotting the estimated effect?

I am trying to model the visitation frequency of bird to a plant species along an elevational gradient and two season. Moreover I have the number of flowers each observed plant had. My response...

02 August 2023 8,341 2 View

What is the histogram for the normal distribution of systolic blood pressure in the US?

I cannot easily find the data about blood pressure expressed in the normal distribution histograms I am best aquainted with. Where can I find them?

22 July 2023 1,321 1 View

If I only get a fastaq file from my whole plasmid sequencing order does it mean that it failed?

Hello, I submitted two samples for whole plasmid sequencing. I usually get a fasta. fastaq, the histogram and the consensus file. This time they only attached the fastaq and the histogram file....

23 May 2023 8,374 3 View

How to plot a histogram with MD simulation output files?

Please I need help, i completed a MD simulation and i want to plot this kind of histogram (the one I have attached). Which software can I use?? Also, what tools can I use apart from VMD to analyze...

21 March 2023 4,747 3 View

Can I use Chi-square test for normally distributed data?

Dear Experts, Can we use the chi-square test (which is non-parametric) on normally distributed data? A follow-up to this is.. can both parametric and non-parametric tests be performed on the same...

19 March 2023 9,932 8 View

Which tool use to plot horizontal histograms as shown in the attached figure?

These types of figures are required in reaction kinetics modeling work. #figure #plot #Histogram #reaction_kinetics #chemical_kinetics #combustion #combustion_kinetics

29 January 2023 480 7 View

Hello, can anyone guide me on how to plot a dose volume histogram (DVH) with Excel values (not DICOM) using MATLAB or Python?

If you know a software for drawing dose volume histogram (DVH) using numbers in any format other than DICOM format, it can be helpful. thank you.

21 December 2022 3,660 13 View

How to calculate the total cellular fluorescence intensity using CytoFLEX Flow Cytometer?

I have the dot plot and the histograms of the samples after the treatment with the fluorescence-labeled nanoparticles.

20 November 2022 1,255 0 View