I tried to lyse the E.coli cell by using sonication. I resuspended the cell pellet in buffer (1X PBS, 0.5% Triton X-100 and protein inhibitor cocktail), left it on ice 30 min and sonicated at 20% amplitude, 20s on/30s off for 7 min. After sonication, the mixture was still turbid, not transparent and the white pellet after centrifugation has the same size as cell pellet. I check the supernatant on SDS-PAGE but the yield is very poor. I tried with higher amplitude to 40%, prolonged the time but the result was not improved. I do not know what is wrong with my lysis process.
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