How can I use McrBC combined with PCR to analysis methylation status of genes?

10 October 2015 0 6K Report

I am going to used methylated sensitive restriction enzymes combine with PCR to analysis methylation status of cytisines in a plant gene. While restriction site of HpaII/HspI can be determined easily, I cannot find any program which can predict McrBC recognized sites. I know McrBC having loose cleavaged position, so how I can determine cleavage pattern of McrBC to design primers for PCR?

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Science method

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