Calcium Retention Capacity(CRC) is one way to measure the MPTP of mitochondria where calcium is added to the sample, eliciting a spike in signal that dissipates due to the uptake of calcium into the mitochondrial matrix until MPTP collapses, which results in release of the accumulated calcium from the matrix, causing a sudden and sustained increase in fluorescence signal. this is clearly seen in organs like heart, liver but for the brain it stays flat instead of increasing and I don't know why..?
Can you give a bit more information on your protocol? The brain has loads of mitochondria so I would have thought you would get a response. Do you see the initial decrease in fluorescence as the mitochondria uptake the calcium, which usually occurs before the increase as the mitochondria release the calcium?
NO I don't have any problem getting a response( pulses in result of Ca2 uptake) but my question is about the ending of the crc or after the MPTP opens in the brain and why does it stays flat after bit of increase compared to other organs. Please see attached for better understanding of what I mean. by the way I am more concentrated on the synaptic mitochondria specifically in the dopaminergic neurons of the substantial Niagara.
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