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Questions related from Fereshteh Sadat Younesi
With treatment of cell lines ( cardiofibroblast cells), changes in mRNA level of the gene of interest has been observed overtly, but there is no change in protein level. I am wondering how this...
08 August 2017 2,035 7 View
It has shown that about gene knock out in mice'brain, the amount of other protein can be affected (Maybe in mRNA level or protein stability). I want to know whether point mutation can lead to the...
05 May 2017 3,810 4 View
I'm looking for a set of books that I can read in preparation for the GRE Subject Test In Biochemistry, Cell And Molecular Biology
09 September 2015 8,085 1 View
I want to know oncology books that is comprehensive. I want to know from beginner to advanced. I need materials that is useful for molecular medicine major.
07 July 2015 9,032 3 View
Article show that rigidity affects enzyme activity and high rigidity causes low activity. Is there any exceptions? How does increasing rigidity cause high activity?
02 February 2015 9,761 11 View
When enzymes were eluted with high concentration immidazole, some of them lost their activity in 2 days. I want to know how immidazole affect enzyme or other protein. I can't find article about that.
01 January 2015 8,631 7 View
my enzyme is cellulase. It has two domains( small domain and catalytic domain). small domain has a few hydrophobic interaction with catalytic domain. I determined PH optimum for truncated and...
12 December 2014 8,588 4 View
I removed small domains from my enzyme. I want to know which one (truncated or wild-type) is more likely to aggregate at different times at 65 centigrade. Can you give me a strategy to go about...
12 December 2014 5,999 9 View
I remove 85 aa from my enzyme(cellulase). I purified enzymes(truncated and wild-type). After dialysis, I run 20 microliter of them for SDS-PAGE. Both of them have the same sharpness on gel, but...
11 November 2014 7,923 4 View
For understanding of the function of domain, I removed small domain ( with 7 beta sheet) from my enzyme. my enzyme( cellulase) has two domain that the catalic domain is major domain. I check...
10 October 2014 544 2 View
I can't purify my protein with the ni-agarose column. The PH wash and elution buffer is ok but all of my protein eluted in the wash buffer. What is my problem?
10 October 2014 1,060 17 View
Is there any solution for removing these globular objects?
09 September 2014 8,989 6 View
I provide mix buffer containing 100 mM glycine, 100 mM tris, 100mM succinic acid and 100 mM imidazole with ph 3, 3.5.....9 25 microlitr enzyme in tris buffer with ph 7.5 was mixed with 50...
09 September 2014 5,297 20 View
I can't understand that how PH cause his tag proteins bind to Ni-agarose resin or come off it. I want to know more about interaction. can anybody suggest a good article about that? for example in...
09 September 2014 9,766 7 View
I deleted a small domain from an enzyme, I understand that its expression is so decreased.
08 August 2014 5,575 9 View
I use Tris-HCL as lysis buffer for thermophilic enzyme. Is it possible the PH of this buffer changes when I use high temperature for enzyme assay?
08 August 2014 5,184 15 View
I don't understand what the relation is between amount of OD and time of induction.For example my recombinant protein is expressed well when I induce bacteria in OD=1. While another protein was...
07 July 2014 2,333 10 View
I need PH optimum for enzyme for doing a good dialysis, so I determined PH and temperature optimum by impure enzyme. Is there significant difference with pure enzyme?
07 July 2014 6,795 7 View
I read some articles that have used ethanol+ solution NaOH for making macro-bead after dissolving chitosan in acetic acid 2%. I don't understand thier reason. I want to use macro-beads for...
06 June 2014 2,507 3 View
I want to simulate seizure and epilepsy in mouse, but I can't find a proper dose of pentylenetetrazole in saline in papers.
06 June 2014 6,160 4 View
My recombinant protein, cellulase, produced in E.coli. I remove small domain from it. I think when my protein produce in high concentration, its activity is reduced.
06 June 2014 9,908 3 View
I purified my enzyme with 250 mM imidazole by ni-agarose column, my enzyme has 2 metal in structure ( Ca and Zn). Is it necessary to add this metal into buffer dialysate?
05 May 2014 1,780 1 View
How much glucose could be effective for optimized expression? And at which stage should I add the glucose to the culture? i want to optimize expression of recombinant pro in pET21a(+). I use...
05 May 2014 8,035 7 View
I purified my protein with 250 mM imidazole, after 2 days, I remove imidazole with dialysis, but I can see a white pellet in protein. My protein is an enzyme, it has activity immediately after...
05 May 2014 6,973 17 View
After staining and destaining my gel, my samples combined with each other and I can't see boundary between our samples
04 April 2014 1,177 12 View
Molecular weight of protein is approximate 55 kDa . I check the different temperature (22, 30, 37) and different time (3, 6, 9... 24). But I can't see a sharp band on SDS-PAGE. When the media...
04 April 2014 4,216 6 View
