Why is my GELMA opaque?

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How to calculate the dissociation energy of the oxides per unit volume?

I have prepared a Erbium Doped Borate glass and I am working on calculations of Elastic Moduli of the glass. I have referred some of the articles but I couldn't understand how they have...

23 May 2024 5,707 0 View

Can someone explain the procedure for Calculating Judd Ofelt Parameters?

I'm working on a project related to Luminescence Properties of Rare Earth Glasses (not Europium). I have read some articles related to Judd Ofelt Parameters, but I couldn't able to understand the...

26 March 2024 2,287 0 View

How do I prepare the sample for an optical microscope in order to obtain droplet size in an emulsion? ?

How do I prepare the sample for an optical microscope in order to calculate droplet size in an emulsion from the image and get a uniform droplet size distribution image?

19 March 2024 7,848 1 View

Why is my GELMA hydrogel degrading in two days ?

Hi, I synthesized GelMA using carbonate buffer and maintained the pH at 9 throughout the synthesis. The carbonate buffer was made with MilliQ water. Subsequently dialysis was done using MilliQ...

28 January 2024 2,010 0 View

How to measure mean exosome size from Nanosight nanoparticle tracking analysis?

Hello, We are looking to measure mean exosome size (range 50 to 150 nm) from Nanosight ns300 nanoparticle tracking data. The output is an Excel file with the average particle concentration at each...

08 January 2024 1,275 2 View

How can one calculate the refractive index from the absorbance data of the glass?

I have conducted an experiment on the UV Absorption Spectrum for my glass samples and I have obtained the Absorbance values for the corresponding wavelengths. Unfortunately, I did not measure the...

17 December 2023 5,071 3 View

Why fcc metal will not undergoes ductile to brittle transition temperature?

the fcc metal are not undergoing DBTT but bcc & hcp will undergoing and how it is happening

10 September 2023 6,549 0 View

How to find oxygen level(ppm) of solid ingot?

explain theory and formula for calculating oxygen level in solid ingot. During pouring of liquid metal into ingot mold, after solidification, forging and heat treatment operation to know oxygen...

06 September 2023 248 0 View

What is delta glaze?

why delta glaze is applying to titanium ingot before starting an forging operation

06 September 2023 8,716 0 View

How anti-flacking cycle is created in heat treatment?

what is the formula is used for creating a anti flacking cycle

20 August 2023 2,116 1 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,478 3 View

Can formaldehyd-fixed gelatin be melt?

I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...

07 August 2024 1,685 1 View

I want to remove Urea from my protein sample?

I want to remove Urea from my protein (6kDa) sample. I did use : 1. Step down the Urea gradient (dialysis) in order to remove urea and refold my protein, but my protein still contains urea in the...

06 August 2024 1,350 4 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,129 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,726 4 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 966 3 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,404 6 View

How are aPTT and PT values reduced after hemodialysis compared to before dialysis value in patients with chronic kidney disease?

I am conducting my research regarding blood coagulation in patients with CKD. I observed a reduction in post hemodialysis aPTT and PT values compared to pre hemodialysis values. But most of...

30 July 2024 3,467 0 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View