Hi Sruthi!

Option 1:

I recommend utilizing SEM analysis to capture images of the droplets, coupled with ImageJ software or the SEM's built-in tools for precise droplet size measurements.

For analyzing droplet distribution, consider employing Dynamic Light Scattering (DLS) analysis. DLS provides a bell curve representation and detailed insights into the size distribution within the sample.

Option 2:

If you want to do it in microscope, keep this as a guide and tweak the procedure

1. Dilute the emulsion if necessary for better droplet separation.

2. Place a small amount of the diluted emulsion on a clean microscope slide.

3. Add a cover slip to flatten and immobilize the droplets, avoiding air bubbles.

4. Seal the edges of the cover slip if needed.

5. Set up the microscope with appropriate magnification and illumination.

6. Capture images of the emulsion droplets.

7. Analyze the images using software or manual methods to measure droplet sizes.

8. Generate a droplet size distribution plot to visualize the uniformity of droplet sizes within the emulsion.

To obtain droplet distribution information from droplet size data:

1. Measure individual droplet sizes.

2. Group sizes into intervals (bins).

3. Count droplets within each interval.

4. Calculate relative frequencies.

5. Plot a histogram or bar chart of droplet sizes.

6. Analyze the distribution shape.

7. Calculate descriptive statistics.

8. Draw conclusions about the droplet size distribution in your sample.

The size distribution of particles in a sample, such as droplets in an emulsion, can often be represented by a probability distribution function.

formula name :log-normal probability density function that relates droplet size, mean of the natural logarithm of the droplet sizes (log-mean), standard deviation of the natural logarithm of the droplet sizes (log-standard deviation).

This formula describes the probability of finding a droplet with a particular size x in the sample. By fitting experimental data to this distribution, you can estimate the parameters μ and σ and characterize the size distribution of the droplets in your emulsion.

I hope it helps