Why I found difficulty in detachment by trypsinization of my adherent cells after selection with drug?

- I want to do expansion for my A549 cells to bigger plate after selection with (Ab) drug there is a lot of drug resistant cells colonies but I lost MOST of them when I tried to harvest them by...

06 July 2018 5,501 1 View

Why are my cells (Beas-2b)not growing nicely!?or what my cells should looks like?

I'm trying to culturing the human bronchial epithelial cells in DMEM high glucose,10%Fcs,100x p/s...But after thawing them from the first frozen stock in -80C I got granulated and vacuolated...

01 February 2018 7,680 7 View

How to determine the proper time and concenterations for treatment experiments!?

If you are treating some cell line with chemical substances for the first time in your experiment should we look for recommended concentrations and exposure time in previous literature or should...

01 January 1970 727 2 View

Should I use seed my stable cell line in complete or selection media for any future treatment experiments?

If I want to perfom treatments for my stable cell line with different concentrations from any chemical compounds! Q1)So should I seed my cells in complete culture medium or selection medium that...

01 January 1970 1,725 4 View

Why my transfected cells demonstrate dull HOECHST 33342 staining!?

Hello Good people When I stained my adherent non-transfected cells with Hoechst 33342 staining it showed blue fluorescence but dull staining happened with GFP transfected cell I used 2ug per...

01 January 1970 894 7 View

Why I got low cell viability after thawing!?

01 January 1970 5,804 10 View

Is this method of freezing applicable to all cells types! ?

Media for freezing of cells for culture. Requirements 700ml RPMI (2mM L-Glutamine) 200ml FCS 100ml DMSO Method Filter sterilize 700ml of RPMI-1640 (2mM L-Glutamine) and 200ml fetal calf serum...

01 January 1970 136 2 View

After How many paasges should I freeze down my stable cells?

- I expanded my A549 stable cells from 12 well plate to 6 well plate should I freeze them down or expand them more in 25cm flask! ? Because I read: Some clones may lose expression after several...

01 January 1970 4,308 13 View

Which programme could I use to calculate transfection efficiency?

I know if I want to calculate transfection efficiency it would be done by this equation LIKE THIS % Transfection efficiency = (number of cells stained with fluorescent positive control dye/total...

01 January 1970 4,439 3 View

Is it possible or realistic to do heat chock treatment for cells on hot plate?

If I donot have a humidified incubator with 42c, is it possible to expose cells growing on movable slides to the hot plate at 42 -42 C for 1 min! I heat shock experiment

01 January 1970 8,142 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

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02 March 2021 3,060 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View