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Questions related from Udesh Dhawan
Dear fellow researchers: I am working on osteosarcoma cells (U2OS) and am looking for ways to block TGF-Beta signaling pathway (towards Oct-4). Has anyone tried anything related to this...
08 August 2019 4,416 6 View
Dear fellow researchers: I need to prepare 1.6% chromic acid for the purpose of AAO etching. The amount of information available online to make chromic acid is overwhelming. I also could not find...
10 October 2018 770 2 View
Dear fellow researchers: Ive been doing this experiments for a while but recently I've observed that not all of my cells are getting stained with FITC-Phalloidin. Can anyone suggest possible...
09 September 2018 6,244 9 View
Dear fellow researchers, I want to stain TGF-B1 in the cells. I haven't done this in the past. If some has, can anyone recommend a good primary Ab company (tried and tested) with good specific...
08 August 2018 6,741 5 View
Dear fellow scientists: I am working on fabrication of nonporous Anodised Aluminum Oxide. I am performing the electrolysis in 0.3M oxalic acid at 40V (CV mode in the power supply). However,...
08 August 2018 7,150 6 View
Dear Fellow Researchers, I am working on the anodization process and for a brief period of time (3 minutes), I need to achieve and maintain the temperature of the solution (oxalic acid) between...
08 August 2018 8,409 2 View
Dear all, I have an organic compound with the difference in chirality. The binding of R-chiralty with Fetal Bovine Serum (FBS) is lower than the binding of S-Chirality with FBS. While I have...
03 March 2018 9,190 12 View
Dear fellow researchers, I am aware of the SRB assay for analyzing viability of cells cultured directly on-to culture dishes. However, my cells are growing on opaque silicon substrates and I want...
03 March 2018 3,700 3 View
Dear fellow researchers, I'll be running some experiments where I will have to analyze the cellular adhesion to different substrates. Can someone recommend a specific technique to quantify the...
01 January 2018 867 9 View
Dear all, I have seen in many research papers that scientists study the expression of a gene in the modified stated as well as in the phosphorylated state after a specific treatment. Can anyone...
07 July 2017 5,296 5 View
Dear fellow researchers, Does anyone have any information or experience regarding resistance of cross-linked PDMS substrate (10-20 microns) to diluted (5-10%) sulphuric, oxalic or phosphoric acid?
06 June 2017 1,565 2 View
Dear fellow researchers, I haven't had any luck in finding any literature which has used Reverse Transcription PCR to analyze vinculin gene expression. Does anyone have any information/experience...
07 July 2016 3,040 3 View
Dear all, I am facing a particular problem while preparing the cell line MDA-MB231 for confocal microscopy. I use 3% paraformaldehyde for fixation. I wash them with serum free medium before...
05 May 2015 2,627 5 View
Dear all, I am facing some confusion while doing a student's t-test using excel. The thing is, I have two variables. One is the different number of days and the second is the different kinds of...
05 May 2015 9,211 17 View
