Doaa Yamani

11 Questions 34 Answers 0 Followers

Questions related from Doaa Yamani

Why I found difficulty in detachment by trypsinization of my adherent cells after selection with drug?

- I want to do expansion for my A549 cells to bigger plate after selection with (Ab) drug there is a lot of drug resistant cells colonies but I lost MOST of them when I tried to harvest them by...

07 July 2018 5,559 1 View

Why are my cells (Beas-2b)not growing nicely!?or what my cells should looks like?

I'm trying to culturing the human bronchial epithelial cells in DMEM high glucose,10%Fcs,100x p/s...But after thawing them from the first frozen stock in -80C I got granulated and vacuolated...

02 February 2018 7,730 7 View

How to determine the proper time and concenterations for treatment experiments!?

If you are treating some cell line with chemical substances for the first time in your experiment should we look for recommended concentrations and exposure time in previous literature or should...

01 January 1970 782 2 View

Why I got low cell viability after thawing!?

As recommended by ATCC I freezed my Calu-3 cell line down(-- 80 °C ) in Complete growth medium supplemented with 5% DMSO but I got low cell viability AFTER just two weeks from freezing them...

01 January 1970 5,860 10 View

Why I got margin cells with strong signals and middle cells with faint signal in ICC?

- When I performed ICC work for treatment cells in translocation stress experiment in IBIDI 12 well removable chamber I got variant signal intensities ... What is the reason behind cells stress...

01 January 1970 2,200 2 View

Which programme could I use to calculate transfection efficiency?

I know if I want to calculate transfection efficiency it would be done by this equation LIKE THIS % Transfection efficiency = (number of cells stained with fluorescent positive control dye/total...

01 January 1970 4,489 3 View

Why my transfected cells demonstrate dull HOECHST 33342 staining!?

Hello Good people When I stained my adherent non-transfected cells with Hoechst 33342 staining it showed blue fluorescence but dull staining happened with GFP transfected cell I used 2ug per...

01 January 1970 955 7 View

After How many paasges should I freeze down my stable cells?

- I expanded my A549 stable cells from 12 well plate to 6 well plate should I freeze them down or expand them more in 25cm flask! ? Because I read: Some clones may lose expression after several...

01 January 1970 4,374 13 View

Should I use seed my stable cell line in complete or selection media for any future treatment experiments?

If I want to perfom treatments for my stable cell line with different concentrations from any chemical compounds! Q1)So should I seed my cells in complete culture medium or selection medium that...

01 January 1970 1,784 4 View

Is this method of freezing applicable to all cells types! ?

Media for freezing of cells for culture. Requirements 700ml RPMI (2mM L-Glutamine) 200ml FCS 100ml DMSO Method Filter sterilize 700ml of RPMI-1640 (2mM L-Glutamine) and 200ml fetal calf serum...

01 January 1970 200 2 View

Is it possible or realistic to do heat chock treatment for cells on hot plate?

If I donot have a humidified incubator with 42c, is it possible to expose cells growing on movable slides to the hot plate at 42 -42 C for 1 min! I heat shock experiment

01 January 1970 8,198 2 View