Why I found difficulty in detachment by trypsinization of my adherent cells after selection with drug?

- I want to do expansion for my A549 cells to bigger plate after selection with (Ab) drug there is a lot of drug resistant cells colonies but I lost MOST of them when I tried to harvest them by...

06 July 2018 5,501 1 View

Why are my cells (Beas-2b)not growing nicely!?or what my cells should looks like?

I'm trying to culturing the human bronchial epithelial cells in DMEM high glucose,10%Fcs,100x p/s...But after thawing them from the first frozen stock in -80C I got granulated and vacuolated...

01 February 2018 7,680 7 View

Why I got margin cells with strong signals and middle cells with faint signal in ICC?

- When I performed ICC work for treatment cells in translocation stress experiment in IBIDI 12 well removable chamber I got variant signal intensities ... What is the reason behind cells stress...

01 January 1970 2,139 2 View

How to determine the proper time and concenterations for treatment experiments!?

If you are treating some cell line with chemical substances for the first time in your experiment should we look for recommended concentrations and exposure time in previous literature or should...

01 January 1970 727 2 View

Should I use seed my stable cell line in complete or selection media for any future treatment experiments?

If I want to perfom treatments for my stable cell line with different concentrations from any chemical compounds! Q1)So should I seed my cells in complete culture medium or selection medium that...

01 January 1970 1,725 4 View

Why my transfected cells demonstrate dull HOECHST 33342 staining!?

Hello Good people When I stained my adherent non-transfected cells with Hoechst 33342 staining it showed blue fluorescence but dull staining happened with GFP transfected cell I used 2ug per...

01 January 1970 894 7 View

Why I got low cell viability after thawing!?

01 January 1970 5,804 10 View

Is this method of freezing applicable to all cells types! ?

Media for freezing of cells for culture. Requirements 700ml RPMI (2mM L-Glutamine) 200ml FCS 100ml DMSO Method Filter sterilize 700ml of RPMI-1640 (2mM L-Glutamine) and 200ml fetal calf serum...

01 January 1970 136 2 View

After How many paasges should I freeze down my stable cells?

- I expanded my A549 stable cells from 12 well plate to 6 well plate should I freeze them down or expand them more in 25cm flask! ? Because I read: Some clones may lose expression after several...

01 January 1970 4,308 13 View

Which programme could I use to calculate transfection efficiency?

I know if I want to calculate transfection efficiency it would be done by this equation LIKE THIS % Transfection efficiency = (number of cells stained with fluorescent positive control dye/total...

01 January 1970 4,439 3 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

What is recommended for solid domain in ANSYS FLUENT?

Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?

01 March 2021 9,785 3 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View