I am doing tissue culture in okra, Hypocotyl and cotyledon were used as explants from 9 days old in vitro grown seedling, I used Activated charcoal at different concentration as an absorbent agent.
The Hypocotyl explant induced callus on culture medium having Activated charcoal, whereas on same medium the cotyledon explant did not induced callus. Moreover the cotyledon explant did not induce callus on any culture media supplemented with activated Charcoal. What’s the reason? Why the explants types respond so differently? Please if any valid references will be better.
Thanks
Dear Muhammad,
You have not mentioned whether you supplemented the medium with growth regulators or not. If cotyledon explants do not respond then change the growth regulator combination for that. Different explants can not produce callus on a single medium. The response varies with the age, nature and type of explants and growth regulator combinations. You have to optimize these first. Then assess other parameters which influence the frequency of callus formation. All the best.
Dear sir
i used combination of NAA + BAP, and 2,4-D + TDZ combination in culture media supplemented with Activated charcoal. The cotyledon ex plant did not give callus response on any PGRs combination on medium with Activated charcoal, whereas hypocotyl induce good callus in some combination of PGRs along with activated charcoal.
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