Muhammad Irshad

11 Questions 10 Answers 0 Followers

Questions related from Muhammad Irshad

What is the criteria for deciding embryogenic and nonembryogenic calli?

I have callus masses some are whitish and compact, while others are yellowish and friable , can i called them embryogenic and nonembryogenic calli. i means callus which do not give shoots can be...

18 September 2019 1,197 1 View

How to measure pot size?

I am going to investigate the influence of pot size on growth and yield of tomato . According to literature review some scientists mentioned the pot size in cm 3, some in cm 2, while some in...

25 April 2019 6,331 8 View

How to calculate total nitrogen of MS media in mM?

In several reports it is mentioned that traditional MS media contains 60 mM Nitrogen, if I wants to make MS media with 50, 40 ,30 and 20 mM total nitrogen, How can I make it, if someone help me...

28 June 2017 5,017 8 View

Why low nitrogen level enhances anthocyanin acccmulation in plant tissue?

It has pastulated that low nitrogen concentration in soil or culture medium enhanced anthocyanin acculation in plant tissue with reduced growth? What are the logic beoynd, some one postulated...

18 May 2017 5,802 4 View

Protocol to germinate Tea seed on MS hard medium?

Tea seed is vey hard to germinate, how can i germinate it on MS hard medium in tissue culture lab please

11 April 2017 8,267 7 View

How to access specific crop production country wise (FAO statistics)?

I am workig on okra crop, I need recent informations about its production world wide how can I find it with refrences .thanks

28 March 2017 9,509 6 View

Callus culture for anthocyinine biosynthesis?

i am interested to culture callus for anthocyanine biosynthesis, should i culture my ex-plants on ordinary used medium e.g auxin and cytokinin or i need some additives in my medium.thanks

30 July 2016 4,019 1 View

Calculation of fertiliser for potted experiment?

if my pot have 2 kg soil, i want to use N-P-K at 120-60-50 kg/ha rate, whereas fertiliser source are (1) Ammonium nitrate 33%N, .(2) Potassium chloride 60% K20, (3)Triple super phosphate 46 %...

28 April 2016 2,472 43 View

Should Asorbic and citric acid be added in MS medium before or after autoclaving?

Two important things about this method confuse me. 1. some of the researcher say ascorbic acid and citric acid are heat sensitive, so cannot be add to MS medium before autoclave. 2. 2nd thing is...

21 February 2016 724 9 View

Why Hypocotyl and cotyledon explants respond differently in term of callus induction to culture media having activated charcoal?

I am doing tissue culture in okra, Hypocotyl and cotyledon were used as explants from 9 days old in vitro grown seedling, I used Activated charcoal at different concentration as an absorbent...

14 February 2016 6,699 2 View

How can i decide that it is the best time for my callus to inoculate it in shooting media?

i am doing tissue culture in okra

30 December 2015 2,384 9 View