20 Questions 21 Answers 0 Followers
Questions related from Prem Kumar G
Dear All, I want to design sgRNA target sequence and insert into scaffold portion already present in pRGEB31 binary vector. Any experimental protocol for that? I want to knock out a disease...
13 June 2018 6,460 2 View
Dear All, Any experienced person in sweet orange tissue culture please tell me which explants will be more responive and productive for in vitro regeneration of sweet orange plant? Thanks in advance.
07 June 2018 9,943 4 View
Dear All, I surprised when i saw Agrobacterium tumefaciens colonies streaked on YEM agar plates continue to grow even after i stored at 4degree celsius. After 1 month the colonies overgrow...
07 April 2017 5,907 11 View
Dear Researchers, I am doing transformation in cotton plant. Now I want to check GFP expression in transformed tissue. Can anyone suggest a standard protocol for preparing tissue sample to...
18 November 2016 9,458 7 View
Dear Researchers, I am regularly preparing 1/2 MS liquid media for transformation protocol. It appears good before autoclaving but often gets precipitated (white in color) after...
17 November 2016 2,231 5 View
Hi everyone, I want to do estimate antioxidant enzymes activity of crude protein extract obtained from salt stress induced cotton seedlings. Can anyone give me standard protocols for SOD,...
13 July 2016 8,404 3 View
Dear Researchers, Is it possible for cotton seedlings (10 days old) to produce antioxidant enzymes in cotyledons and other parts under short duration (6h,12h and 24h) of salt and drought...
13 July 2016 8,581 3 View
i have 2 years old callus obtained from cotton. eventhough im not observing any embryo stages im suspecting somatic embryo formation in callus. but i don't know how to confirm that. any suggestion...
04 May 2016 1,877 4 View
Dear all, I have planned to study differential expression of proteins through 2-D electrophoresis (not any target protein) in 7 days old cotton seedlings under different abiotic stress (NaCl,...
19 April 2016 4,197 6 View
Dear Researchers, I was preparing to run 2nd dimension (SDS PAGE) after finishing 1st dimension (isoelectric focusing). After finishing everything perfect, i was sealing top of the IPG strip...
07 April 2016 9,015 12 View
I collected plant samples after stress treatment and immediately ground the samples in liquid nitrogen. then i stored those ground samples at -80degree Celsius. now i want to analyze the protein...
21 March 2016 5,969 2 View
Dear researchers, I have planned to carry out stress treatment under in vitro condition on half strength MS medium supplemented with NaCl and PEG6000. Should I add NaCl and PEG6000 into the medium...
31 January 2016 1,834 2 View
I came across some articles stating that vacuum infiltration resulted higher transformation frequencies in plants. Does anyone have idea about the role of vacuum infiltration in efficient...
18 January 2016 8,237 4 View
I have a protocol for preaparation of tris-buffered phenol for leaf protein extraction. in that protocol it is mentioned to prepare tris-buffered phenol from crystalline phenol. but i have...
06 October 2015 6,103 4 View
I want to prepare tris buffered phenol for protein extraction from plant tissue. But i dont have standard procedure to prepare that. Does anybody have protocol regarding this? Please help me....
23 September 2015 5,022 2 View
i want to confirm whether my cotton callus is embryogenic or not simply through light microscope observation. pls suggest me a good protocol (i.e.staining) for that. is it enough to confirm the...
17 June 2015 5,545 6 View
I got friable embryogenic callus form hypocotyl explant of cotton on MSB5 medium supplemented with 2,4-D and kinetin and got well proliferation with TDZ+IBA. After proliferation, i transferred the...
27 May 2015 1,063 6 View
i did cDNA synthesis from RNA sample using cDNA synthesis kit. can anyone tell how to confirm whether the cDNA is synthesized or not?
26 May 2015 6,995 10 View
i got multiple shoots from cotyledonary node explants of cotton. basal callus formed at basal part of the explant. i did 2 subcultures along with the basal callus into fresh medium. after 2...
26 May 2015 2,497 3 View
Dear Researchers, Can I use real time PCR primers for semi-quantitative PCR to study specific gene expression? Will it give the expression pattern more or less similar to the real time PCR...
01 January 1970 4,366 3 View
