Prem Kumar G

8 Questions 13 Answers 0 Followers

Questions related from Prem Kumar G

How to design and insert my target sequence into sgRNA scaffold?

Dear All, I want to design sgRNA target sequence and insert into scaffold portion already present in pRGEB31 binary vector. Any experimental protocol for that? I want to knock out a disease...

13 June 2018 6,359 2 View

Can bacteria grow even after storing at 4degree?

Dear All, I surprised when i saw Agrobacterium tumefaciens colonies streaked on YEM agar plates continue to grow even after i stored at 4degree celsius. After 1 month the colonies overgrow...

07 April 2017 5,852 11 View

Standard protocol for plant tissue preparation to visualize GFP?

Dear Researchers, I am doing transformation in cotton plant. Now I want to check GFP expression in transformed tissue. Can anyone suggest a standard protocol for preparing tissue sample to...

18 November 2016 9,394 7 View

Why precipitation in MS liquid media?

Dear Researchers, I am regularly preparing 1/2 MS liquid media for transformation protocol. It appears good before autoclaving but often gets precipitated (white in color) after...

17 November 2016 2,181 5 View

Protein expression in cotton seedlings under abiotic stress?

Dear all, I have planned to study differential expression of proteins through 2-D electrophoresis (not any target protein) in 7 days old cotton seedlings under different abiotic stress (NaCl,...

19 April 2016 4,148 6 View

Does bubble formation affect separation of proteins in 2D electrophoresis?

Dear Researchers, I was preparing to run 2nd dimension (SDS PAGE) after finishing 1st dimension (isoelectric focusing). After finishing everything perfect, i was sealing top of the IPG strip...

07 April 2016 8,967 12 View

How can I get somatic embryo from embryogenic callus of cotton?

I got friable embryogenic callus form hypocotyl explant of cotton on MSB5 medium supplemented with 2,4-D and kinetin and got well proliferation with TDZ+IBA. After proliferation, i transferred the...

27 May 2015 1,017 6 View

Will Real time primers work for semi quantitative PCR?

Dear Researchers, Can I use real time PCR primers for semi-quantitative PCR to study specific gene expression? Will it give the expression pattern more or less similar to the real time PCR...

01 January 1970 4,303 3 View