Could someone explain why cyclic-RGD has been used for cell adhesion in tissue engineering studies? I could never find a linear RGD peptide that has been used for cell adhesion in tissue engineering work.
Shorter linear polypeptides have quite flexible terciary structure, which makes them unstable. Receptors like integrins are usually recognising the 3-dimensional (3D) structure of the peptide ligands which is effective when it has a stable terciary structure. If you use cyclic polypeptide, it stabilises the peptide`s 3D structure in space, so that the (integrin) binding will be more effective.
@Prof. Jorg Fielder I have found only linear RGD peptides been used in Gne Delivery or some drug delivery applications. Recent publications towards tissue engineering using RGD (only incase surface functionalization for the cell-adhesive. some authors have used RGD sequence containing amphiphilic peptides as self assembled nanofibers and tat was been used for tissue engineering applications) is only of cyclic RGD
@Prof. Christopher ---- My major doubt is, RGD which is in ECM is of cyclic or linear?
Both linear and cyclic forms of RGD are widely used in TE. The reason many use cRGD is because it has ~240 times higher affinity for binding to some integrin receptors than the linear form, but I'm sorry, I can't remember the source of that information right now. In short, even though it's not naturally occurring, some integrins bind to the cyclic form more readily and it is therefore very useful in TE studies.
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