Domokos Bartis

2 Questions 5 Answers 0 Followers

Questions related from Domokos Bartis

Any tips for critical parameters in the Gateway LR reaction?

I'm struggling with the LR reaction for a long time. 1. I'm using the pENTR4 entry plasmid with a human MKK5 insert - it is sequenced and OK. 2. I'm using pLIX_403 destination vector (Addgene...

06 December 2016 2,112 5 View

What transfection reagent would you recommend for human primary endothelial cells?

Hi, what transfection reagent would you recommend for human primary endothelial cells? I have tried Lipofectamine 2000 on EA.hy926 cell line and on HUVEC, and it seems to be very toxic and the...

04 September 2015 3,653 4 View