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I'm struggling with the LR reaction for a long time. 1. I'm using the pENTR4 entry plasmid with a human MKK5 insert - it is sequenced and OK. 2. I'm using pLIX_403 destination vector (Addgene...
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Hi, what transfection reagent would you recommend for human primary endothelial cells? I have tried Lipofectamine 2000 on EA.hy926 cell line and on HUVEC, and it seems to be very toxic and the...
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