Good day, may I know why my colony for L. Lactis looked watery on agar plate after undergoes fermentation? Thank you.
Good day, may I know if I had 8Kda and 56Kda protein can I used the sds gel protocol that is suitable for 8Kda one for 56Kda protein as well? Or I need to do 2 different formulae for these 2...
04 May 2019 1,665 2 View
Hi. May I know how much in percentage of both stacking gel and separating gel for protein size around 56kDa? Thank you.
03 April 2019 8,159 3 View
Good day, I would like to know more on tips and precautions for fermentation step in yogurt drink (such as YAKULT). If can provide any supportive journal, then is much more better. Also, is there...
05 January 2019 4,275 2 View
31 December 2018 3,645 2 View
Good day, my culture was mesophile and anaerobic. May I know which plate will be suitable for it? Thank you.
23 December 2018 1,362 3 View
Good day, I got cfu count for 1.8 x 10^8 in 10^-5, 1.3 x 10^9 in 10^-6 and 5.7 x 10^9 in 10^-7 in my triplicate serial dilution for L. lactis subsp. cremoris. Although the cfu count per plate was...
11 December 2018 6,617 0 View
11 December 2018 3,944 4 View
Good day. I am looking for standard growth curve for L. lactis subsp. Cremoris (NZ9000) of 600 nm optical density versus 10^7 cfu/ml with. Purpose is to see when this LAB reaches its' log phase....
11 December 2018 1,163 12 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...
06 August 2024 662 1 View
I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...
04 August 2024 5,228 4 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...
01 August 2024 2,250 2 View
Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...
01 August 2024 6,134 2 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...
25 July 2024 2,532 3 View