Recommendation on sds gels (stacking and separating gel) for different size of protein?

Good day, may I know if I had 8Kda and 56Kda protein can I used the sds gel protocol that is suitable for 8Kda one for 56Kda protein as well? Or I need to do 2 different formulae for these 2...

04 May 2019 1,621 2 View

Percentage used in separating and stacking gel in SDS page?

Hi. May I know how much in percentage of both stacking gel and separating gel for protein size around 56kDa? Thank you.

03 April 2019 8,121 3 View

Any tips and precautions for fermentation of yogurt drink?

Good day, I would like to know more on tips and precautions for fermentation step in yogurt drink (such as YAKULT). If can provide any supportive journal, then is much more better. Also, is there...

05 January 2019 4,242 2 View

Any tips and precautions for fermentation of yogurt drink?

Good day, I would like to know more on tips and precautions for fermentation step in yogurt drink (such as YAKULT). If can provide any supportive journal, then is much more better. Also, is there...

31 December 2018 3,607 2 View

Pour plate or spread plate?

Good day, my culture was mesophile and anaerobic. May I know which plate will be suitable for it? Thank you.

23 December 2018 1,322 3 View

Pour plate or spread plate?

Good day, my culture was mesophile and anaerobic. May I know which plate will be suitable for it? Thank you.

11 December 2018 3,906 4 View

Why cfu count is higher when the dilution factor increased?

Good day, I got cfu count for 1.8 x 10^8 in 10^-5, 1.3 x 10^9 in 10^-6 and 5.7 x 10^9 in 10^-7 in my triplicate serial dilution for L. lactis subsp. cremoris. Although the cfu count per plate was...

11 December 2018 6,525 0 View

Microbiology (CFU counting by OD value)-L. lactis subsp. Cremoris (NZ9000)?

Good day. I am looking for standard growth curve for L. lactis subsp. Cremoris (NZ9000) of 600 nm optical density versus 10^7 cfu/ml with. Purpose is to see when this LAB reaches its' log phase....

11 December 2018 1,104 12 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View