Good day, may I know if I had 8Kda and 56Kda protein can I used the sds gel protocol that is suitable for 8Kda one for 56Kda protein as well? Or I need to do 2 different formulae for these 2 different protein size? Thank you.
Please choose the appropriate gel concentration according to the molecular weight of the target protein. For 8Kda protein, it is recommended to use a separation gel with a concentration of 15%, while for 56Kda protein, use a separation gel with a concentration of 10%.