Pour plate or spread plate?

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Robert Thomas Bachmann

Pour plate.

If you have an anaerobic chamber you can also use spread plate. Incubate in anaerobic jar.

For SRBs semisolid Postgate E medium in Bijou bottles is used.

Tun Tt

Robert Thomas Bachmann thanks, may I know how about the selection of plate for facultative anaerobes and mesophile?

In the case of facultative bacteria both spread plate and pour plate should be ok. Just ensure you incubate at ~35°C.