How to visualize the external background charges in gaussview?

I am applying background charges in my sp calculation. To be sure that I am placing them in the right coordinates, Is there any way to visualize the background external point charges in Gaussview?...

11 November 2020 6,060 3 View

What media can be used for real-time H2O2 measurement (intracelular)?

What media or salt solution (HBSS, EBSS, Tyrode) can be used for real-time intracelular h2o2 imaging? I am using a genetically-encoded biosensor based on roGFP and have to find the media that...

28 October 2020 7,301 2 View

How to calculate charge transfer integral (J) in a molecule (intramolecular)?

I want to calculate charge transfer integral to transfer one charge from one part of the molecule to another part. what are the proper ways to calculate this? And which software package is good to...

12 November 2019 6,520 3 View

What is the best method (in HF/DFT/CDFT) to calculate the off-diagonal coupling elements in intra-molecular electron transfer?

What is the best method (in HF/DFT/CDFT) to calculate the off-diagonal coupling elements H_12 for electron/hole transfer in a molecule (intra-molecular electron/hole transfer) ? I have used...

22 October 2019 6,988 5 View

Is there any method based on Koopman's theorem to calculate the off-diagonal coupling elements in intra-molecular electron transfer?

Is there any method based on Koopman's theorem to calculate the off-diagonal coupling elements H_12 for electron/hole transfer in a molecule (intra-molecular electron/hole transfer)

21 October 2019 2,705 9 View

Are there any lexicon-based sentiment analysis frameworks that have been published recently?

I need to find several sentiment analysis frameworks that utilize the lexicon-based approach for my dissertation. Please recommend me some articles related to this topic.

28 May 2019 2,874 4 View

Please tell me which cloning vector should i use for Staphylococcus sciuri.. its urgent

I need to do transformation in Staphylococcus sciuri. I have three vectors in my lab ptz57/RT , pgemt easy and pet101/D TOPO vector. Which vector i can use

13 July 2017 7,051 2 View

How can you merge two files in SPSS when the cases are not perfectly the same?

For one of our replication projects, we have two SPSS datasets. As they are part of a follow up study (we have wave 1 and wave 3), they principally include the same cases. However, the second...

19 April 2013 9,911 44 View

Idea exchange

Hello all, thanks you all for joining the group. As you know, we have come together in this group to discuss and exchange new ideas. I think it is better if we start by a short introduction...

06 June 2009 5,909 4 View

Hello, Is there some experimental results and links about effect of salicylic acid in potato?

Potato is a potential species for exogenous application of salicylic acid and derivates for stress control?

01 January 1970 4,790 4 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View