What media or salt solution (HBSS, EBSS, Tyrode) can be used for real-time intracelular h2o2 imaging? I am using a genetically-encoded biosensor based on roGFP and have to find the media that wouldn't have much buffering capacity as well as compounds acting like antioxidants, since it is important to see how the cells alone are coping with h2o2 adition.

Estimated duration of the experiment - 1-1.5 h.

Cells: ipsc-derived neurons. Standart Neurobasal+B27 media was designed to protect neurons from any kind of oxidative damage and contains loads of antioxidants, so h2o2 don't get the chance to get inside the cell.