It sounds like you are very new to molecular biology. You will need to do a LOT of basic background reading to understand your project. Talk with your lab mentors and go read an introductory biology textbook about the structure of DNA.
I am not new in this field and I know the answer. I asked this question for people who need to learn about DNA especially those whose major not in biology. You should not give such an answer and you do not have to react with my question if you do not like it.
Why is DNA replication performed in the 5' to 3' direction?
DNA replication goes in the 5' to 3' direction because DNA polymerase acts on the 3'-OH of the existing strand for adding free nucleotides. Is there any biochemical reason why all organisms evolved to go from 5' to 3'?
Are there any energetic/resource advantages to using 5' to 3'? Is using the 3'-OH of the existing strand to attach the phosphate of the free nucleotide more energetically favorable than using the 3'-OH of the free nucleotide to attach the phosphate of the existing strand? Does it take more resources to create a 3' to 5' polymerase?
Prof. Allen Gathman has a great 10-minutes video on Youtube, explaining the reaction of adding nucleotide in the 5' to 3' direction, and why it doesn't work the other way.
Briefly, the energy for the formation of the phosphodiester bond comes from the dNTP, which has to be added. dNTP is a nucleotide which has two additional phosphates attached to its 5' end. In order to join the 3'OH group with the phosphate of the next nucleotide, one oxygen has to be removed from this phosphate group. This oxygen is also attached to two extra phosphates, which are also attached to a Mg++. Mg++ pulls up the electrons of the oxygen, which weakens this bond and the so called nucleophilic attack of the oxygen from the 3'OH succeeds, thus forming the phospodiester bond.
I appreciate your concern Aamal Ghazi Mahdi Al-Saadi regarding for people who need to learn about DNA especially those whose major not in biology. Since, I'm an engineering undergraduate, these kinds of simplified answers relating to the biology are really helpful to learns the basics.