Why do we use Amino Acids in culture medium?

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In Gstreamer pipeline Camera distortion correction, from C++ API, I could not able parse camera parameters and not corrected image.?

Hi all, In Gstreamer pipeline Camera distortion correction, From terminal I am able to get distortion corrected image. But from C++ API, I could not able parse camera parameters and not getting...

14 January 2024 781 0 View

Ground Motion Modification with amplitude-scaled method based on ASCE7-22 ?

Hello In ASCE7-22 chapter 16 in 16.2.3.2 Amplitude Scaling section written that “For each horizontal ground motion pair, a maximum-direction spectrum shall be constructed from the two horizontal...

06 November 2022 529 0 View

Can I grow HEK293FT cells in suspension culture?

My lab usually grows HEK293FT cells in an adherent monolayer culture. However, I wanted to try and increase our recombinant protein yield by growing the cells in suspension. I think this is...

14 July 2022 5,896 0 View

How can I Model BRB and define it's Inelastic data in Etabs2019?

I want to design a building with BRB. for modeling BRB in it I have two kinds of sections ("core brace" and "star seismic") when I use "star seismic" and define "Area of yielding core", after...

10 October 2021 2,972 1 View

Did anybody use Labchart sofware for calculating power spectrum for EEG waves ?

I'm trying to interpret my EEG data on rats using Labchart 7 program but i cannot seem to know how to calculate the power spectrum knowing that i watched all related videos on their website. Any...

05 October 2021 6,453 3 View

Which renewable energy source has the greatest potential in developing countries?

Research in renewable energy is far advanced. Technologies are being developed but which renewable energy source will dominate in developing countries in future?

08 September 2019 2,228 15 View

How can we avoid the paradox of plenty and use revenues generated from oil production to improve the socio-economic conditions of local people ?

Socio-economic conditions of local people in most oil-bearing communities in developing countries remain fundamentally deplorable, without good roads, health facilities, schools and teachers,...

06 August 2019 2,760 2 View

How do we overcome food-fuel conflict in the pursuit of biomass for energy production in poor rural communities?

Most poor rural communities especially in third world countries are traditionally oriented towards agriculture. This makes biomass resources easily accessible in such areas although food can...

27 July 2019 2,258 4 View

How to convert a GUI (matlab) to .exe (standalone)?

I need to convert the GUI code to .exe file (for installation)

18 February 2019 8,544 2 View

Can Hydrogen gas be captured and stored using zeolite?

Which type of zeolite can be effectively used for capturing and storing of Hydrogen gas?

10 November 2018 3,914 8 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How Social Media Affects Your Mental Health ?

How Social Media Affects Your Mental Health

04 August 2024 6,961 3 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View