Which renewable energy source has the greatest potential in developing countries?

Research in renewable energy is far advanced. Technologies are being developed but which renewable energy source will dominate in developing countries in future?

08 September 2019 2,066 15 View

How to convert a GUI (matlab) to .exe (standalone)?

I need to convert the GUI code to .exe file (for installation)

18 February 2019 8,347 2 View

Can Hydrogen gas be captured and stored using zeolite?

Which type of zeolite can be effectively used for capturing and storing of Hydrogen gas?

10 November 2018 3,747 8 View

What should i do in order to calculate my purification purity?

Hello.I have purified my protein with Ni-NTA coulmn and i have run my purified fractions on SDS-PAGE but i don't know how to calculate the purity of may bands. how should i do this?Thanks in advance.

17 July 2017 7,928 6 View

Is it possible that a produced recombinant protein shows no activity?

04 October 2016 4,283 7 View

I am new to HLA typing and related work. Could anyone show me precisely how I can compare my HLA class I alleles with those of other populations?

I have HLA-A, -B and -C alleles and frequency data and wish to do multiple comparisons

03 August 2016 1,566 4 View

Problem with SDS-PAGE Running? protein's bands dont migrate fully?

Hello dears. I did run SDS-PAGE gel yesterday, after staining the gel and destaining the gel i saw that protein's bands did not migrate to the end of the gel and they have just migrated half of...

27 July 2016 6,829 6 View

Which method is good for periplasmic lysis of Escherichia coli?

Hello which methods are good to disrupt the E.coli periplasmic space to extract protein and what is the mechanism of each method? Thanks in advance.

05 January 2016 8,825 5 View

Why do both NTC and RT- give the same band size when I run the gel after the Taq- PCR?

02 November 2015 6,255 1 View

Can't see my western blot bands ?

I'm using the same samples, antibody and solutions for my western blot. Previously I was able to see the bands very beautifully but now I can't see any thing. I'm doing the same thing as before...

19 September 2015 6,187 26 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View