11 Questions 17 Answers 0 Followers
Questions related from Nii Sama
Hello I have expired Ni-NTA resin(Exp: 2012/5) and i want to use this resin for my purification process. i want to know that if expiration date will effect on the binding capacity of Ni-NTA and...
21 March 2018 4,648 4 View
Hello dears. I have a question about buffers in protein works i hope that you will help me with this. Why do we use a combination of buffers(it is better to say amphoteric compounds) and their...
14 February 2018 1,126 4 View
Hello. I want to make a Bradford reagent but i have read that this reagent colud be red when it is cationic and blue when it is Anionic and green when it is neutral. What is the effect of these...
02 September 2017 5,925 6 View
Hello.I have made a Bradford reagent and i want to know if my standard curve of my reagent is correct. I have made an standard curve with this concentration of BSA (with the final volume of 0.5ml...
29 August 2017 3,753 4 View
Hello.I have purified my protein with Ni-NTA coulmn and i have run my purified fractions on SDS-PAGE but i don't know how to calculate the purity of may bands. how should i do this?Thanks in advance.
18 July 2017 8,122 6 View
HelloI have purified my protein by Ni-NTA column chromatography. i've used gradient elution in elution step. after collecting the fractions i should read the bradford absorption of fractions to...
18 February 2017 6,695 3 View
Hello I want to work on production of a recombinant protein(recombinant human growth hormone) in a laboratory and the answer of this question is very important to me. Is it Possible that after...
05 October 2016 4,422 7 View
Hello dears. I did run SDS-PAGE gel yesterday, after staining the gel and destaining the gel i saw that protein's bands did not migrate to the end of the gel and they have just migrated half of...
28 July 2016 6,949 6 View
Hello Why do we use Amino Acids in culture medium, i mean what is the mechanism of used amino acids in molecular point of view? What is they role in cell mechanism? any publication? Thanks in...
02 February 2016 2,335 3 View
Hello what is the average coefficient for changing wet weight of E.coli cells to dry weight? and what is the average weight of each OD for E.Coli cells? Thanks in advance.
25 January 2016 6,154 4 View
Hello which methods are good to disrupt the E.coli periplasmic space to extract protein and what is the mechanism of each method? Thanks in advance.
06 January 2016 8,935 5 View
