Does pluronic F-127 make surfaces hydrophobic?

A plasma bonded PDMS device to glass slide when treated with pluronic, will make the surface hydrophobic? I know that pluronic is used to avoid deposition on surfaces, but what does it really do...

31 December 2019 2,341 2 View

Why is two step immunostaining better than one step immunostaining?

I am currently using a direct immunostaining method to stain my cells, upon using antifade, I can only see DAPI and nothing else. So, is two step immunostaining better than one step?

11 December 2019 1,371 3 View

Do microfluidic devices start degrading in cell culture media over more than a week's period of time?

I am trying to culture cancer cells in microfluidic device, but after 72 hours, the device starts to look fuzzy under the microscope. What is causing this fuzziness? Thank you in advance.

07 August 2018 1,808 3 View

Is Annexin V 10X buffer harmful for cells?

If I use the buffer without dilution, would it kill the cells?

04 May 2018 750 1 View

Why does intensity of an image containing fluorescence increases, with increasing dilution?

Co is the initial concentration of the dye, while Cn is the concentration of the dye after dilution. Intensity of the images are found using ImageJ as well as MATLAB, there is hardly any...

05 June 2017 3,714 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View