Adity Pore

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Questions related from Adity Pore

Does pluronic F-127 make surfaces hydrophobic?

A plasma bonded PDMS device to glass slide when treated with pluronic, will make the surface hydrophobic? I know that pluronic is used to avoid deposition on surfaces, but what does it really do...

01 January 2020 2,446 2 View

Why is two step immunostaining better than one step immunostaining?

I am currently using a direct immunostaining method to stain my cells, upon using antifade, I can only see DAPI and nothing else. So, is two step immunostaining better than one step?

12 December 2019 1,436 3 View

Why do we add sodium pyruvate and penicillin to DMEM cell culture media?

Why does penicillin not stop contamination in cells if it acts as an antibiotic?

11 November 2018 4,020 1 View

Do microfluidic devices start degrading in cell culture media over more than a week's period of time?

I am trying to culture cancer cells in microfluidic device, but after 72 hours, the device starts to look fuzzy under the microscope. What is causing this fuzziness? Thank you in advance.

08 August 2018 1,868 3 View

Is Annexin V 10X buffer harmful for cells?

If I use the buffer without dilution, would it kill the cells?

05 May 2018 807 1 View

Why does intensity of an image containing fluorescence increases, with increasing dilution?

Co is the initial concentration of the dye, while Cn is the concentration of the dye after dilution. Intensity of the images are found using ImageJ as well as MATLAB, there is hardly any...

06 June 2017 3,787 4 View