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Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
Hello I want to know about the sputtering condition of depositing FTO from target 95:5%. I tried with RF sputter in pressure 2.5Pa and 100 sccm Ar at room temperature but it showing no...
21 July 2024 1,680 2 View
Hi everyone! I am working with bacteria and fungal collected from marine sediments. I would to know the methodology since the preparation of biomass until the extraction and stored of secondary...
03 July 2024 6,177 3 View
I made a series of jump tests in subjects, everything was recorded with Vicon. I have data from markers and force plate. We recorded the tests before and after an intervention. My question is, I...
23 June 2024 9,381 4 View
One of the most intriguing frontiers in contemporary research is the application of Generative Artificial Intelligence (GenAI) in qualitative research. This technological advance offers tools that...
13 June 2024 781 2 View
Hello there. Several references say that there is a reader for .sav files from SPSS in Weka, but I just can´t find it. Can anyone provide help? Thanks in advance
12 June 2024 3,844 2 View
Hello esteemed colleagues, I am reaching out to the research community to gather insights on the latest and emerging qualitative research topics in the field of supply chain management. As we all...
06 June 2024 2,406 1 View
Hello, fellow researchers, I am currently exploring the field of supply chain management, with a particular interest in procurement and supplier relationship management. I am keen to understand...
06 June 2024 5,800 0 View
The terms Fictional Language, Fictitious Language, Artificial Language, or Constructed Language in fiction has been used interchangeably in papers that I have read. Are there differences in these...
05 June 2024 3,453 7 View
I was wondering if the plasma that is in the top layer after centrifugation can be used to assess proteins, metabolites, hormones, or other molecules, or if the ingredients of the Ficoll alter the...
03 June 2024 1,852 4 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...
06 August 2024 977 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
