Hi! I would like to know why my agarose gel look like this. I'm trying to extract genomic DNA from hole blood. I put 15, 30, 50 and 100 ng of this DNA and I ran my samples at 100 V for 50 min.
Why are you running out gDNA on an agarose gel? Just continue to the next step in your protocol.
The quality of your dna is excellent with almost no degradation so is suitable for any downstream techniques. If you are worried about the small amount of sample splitting on samples 2 and 4 I would expect this to happen if either the gel was not fully set when the comb was removed causing a rough edge to the well or that there is some accumulated dirt on the comb transferring onto the well wall as the gel sets. Washing the comb with soapy water may help if this is the case
Do you want the clear and crisp bands means u may reduce the ng of your sample like 2,4,6 so on.
Mónica Itzel Martínez Gutiérrez
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