Mónica Itzel Martínez Gutiérrez

4 Questions 1 Answers 0 Followers

Questions related from Mónica Itzel Martínez Gutiérrez

Isolation of PBMC from buffy coat using Ficoll?

Hi! I'd like to know if someone has advices or a protocol for isolating PBMCs from buffy coat (buffy coats from blood banks that contain lymphocytes, monocytes and granulocytes already separated...

last month 2,956 1 View

Fc blocking alternatives in FACS?

Hi! I will isolate B cells from human peripheral blood and I have to use a Fc blocker. Using 1% BSA or 1% FCS in PBS as a staining buffer will be enough? Is better to use a comertial blocking...

14 March 2025 7,578 3 View

Why do my agarose gel look like this?

Hi! I would like to know why my agarose gel look like this. I'm trying to extract genomic DNA from hole blood. I put 15, 30, 50 and 100 ng of this DNA and I ran my samples at 100 V for 50 min.

29 January 2025 406 3 View

Hello, how do I calculate sample size in a study that will compare antibody titles and its specificity between 2 b cell types?

The experiment consist in testing 2 types of b cells with different stimuli and measure the antibody title concentration after 3 days of culture. I don´t know how to calculate how many people do I...

31 October 2023 2,246 1 View