Today, the most commonly used techniques for assessing the concentration of EV samples are NTA and the determination of total protein concentration. However, the concentration obtained by NTA includes all particles (including contaminants), whereas total protein concentration is only an indirect measure to estimate the EV concentration.
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The concentration of CFSE+ particles in extracellular vesicle (EV) samples could potentially provide a more accurate measurement of EV concentration compared to currently used techniques such as nanoparticle tracking analysis (NTA) and total protein concentration determination. NTA measures the concentration of all particles present in the sample, including contaminants, which may lead to overestimation or inaccurate representation of true EV concentration. On the other hand, total protein concentration is only an indirect measure of EV concentration and may not accurately reflect the number of EVs present.
Using CFSE+ particles specifically as a marker for EVs allows for more targeted and specific detection of EVs within the sample. CFSE (carboxyfluorescein succinimidyl ester) is a fluorescent dye that can be incorporated into EV membranes, providing a reliable and quantifiable signal for EV detection. By measuring the concentration of CFSE+ particles, researchers can more accurately assess the true concentration of EVs in the sample while minimising interference from contaminants or non-EV particles.
To best answer this question, it is important to acknowledge the limitations of current EV concentration measurement techniques and highlight the potential advantages of using CFSE+ particles as a marker for EVs. Additionally, it would be beneficial to discuss any validation studies or comparisons with established methods that demonstrate the accuracy and reliability of CFSE+ particle measurement for EV concentration determination.
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