Why do MTT and XTT assays give inconsistent results?

More Jakub Wojcieszak's questions See All

7-Azido-4-Methylcoumarin (7-AzidoMC) as a fluorescent probe for detecting hydrogen sulfide (H2S)?

How effective is 7-Azido-4-Methylcoumarin (7-AzidoMC) as a fluorescent probe for detecting hydrogen sulfide (H2S) in various brain samples such as cell plasma, tissue homogenates, and stained...

03 April 2024 5,389 0 View

What tools are currently available to predict operons in bacterial genomes?

Hi everyone, I was looking for some tools (preferably web-based tools) to predict genes organized in operons in various bacterial sequences I currently work on (plasmids, chromosoms, etc.). For...

19 February 2024 8,922 2 View

What are the recepies for Aerogel synthesis?

I am looking for new recepie /formulas for Aerogels synthesis, and new cooperation in this field.

31 December 2023 8,121 0 View

Why does viability in chondrocytes not decrease after treatment with LPS?

Hello, I have been exposing human chondrocytes to LPS (Lipopolysaccharides from E. coli O111:B4) in concentrations from 1 µg/ml to 40 µg/ml for 24 h and 48 h and haven’t seen any decrease in cell...

16 November 2023 5,297 2 View

Why during my compression tests the stress-strain curve is concave?

I was doing the compression tests on previously thermomechanically deformed pure titanium. The samples were uniaxially compressed in room temperature. The samples were cylindrical, and their...

04 October 2023 6,826 1 View

What is the state of art of the machine learning and AI in the design of experiment?

Is the machine learning and AI in the experimental design a thing?

25 June 2023 4,822 2 View

Are there scales, check lists or other methods to assess the quality and transparency of research using patient data (especially imaging)?

Are there methods to evaluate studies using medical data?

06 June 2023 9,384 3 View

How to measure the Minimal Detectable Change?

Hello, at this moment I am doing an literature overview in topic of clinical studies about viscosupplementation in OA patients. I am interested in the possibilities to count the Minimal...

01 March 2023 2,486 3 View

Is knowledge of biological age essential for strength training in soccer during puberty or can we train safely without this information?

How can information about the biological age of elite players be used in strength training during puberty? Can strength training be individualized with respect to the peak high velocity? Can...

28 January 2023 3,875 3 View

How do you deal with cytotoxicity in a factorial design?

Hello. I would like to ask, how should I incorporate the cytotoxicity as a response in the factorial design, since cytotoxicity depends on concentration? My only idea is to evaluate the IC50 for...

04 December 2022 8,045 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View