Hi everyone!

Lately I've been running into the problem that in some lanes in my SDS-page gel, the sample is not running correctly (see picture) . In these cases the rest of the gel, based on ponceau staining and western blot, is completely fine. In the effected lanes, I can still detect my protein around the expected size on western blot but as a dot rather then as a normal band.

I've already tried better mixing the components of the gel before casting it.

We run 8% gels with bis-tris in 1x mops. I've never had this issue before when preparing the gels with tris-HCL but switching back to that is unfortunaletly not an option.

Does anybody know what I'm doing wrong and/or how to solve this issue? Thank you in advance!