Why crude fibre is present in protein isolates?

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How can I do photodetector application from Inorganic nanoparticles compound?

I have an inorganic nanoparticle compound I want to do a photodetector application from this compound, I do not have any idea, can anyone help me with this?

11 July 2024 135 1 View

There is a thing going on to give the international award for money, they ask money and give awards, is this legal?

The website is https://researchgateway.in/award_reg?id=317 A friend came through this and asked me if this is authentic. When I researched, I found this is a market research company and giving...

14 June 2024 4,368 5 View

How can I run Desmond simulation software on my institute's HPC?

I have Desmond running on my local pc but would like to use the resources of HPC facility. How do I install it on HPC and how to then link it to my pc for using HPC CPU and GPU for speedier...

30 April 2024 6,950 0 View

Acceptable Range of difference of two isoelectric point?

Hi All, I have a following question: How much difference of isoelectric point between a wild type and mutant protein ca be accepted? E.g. The isoelectric points of my target wild type protein...

01 April 2024 1,847 0 View

Is there a way to add single-item measure in confirmatory factor analysis?

I'm conducting a confirmatory factor analysis and one of my measures has only one item. I'm not able to run the analysis when including that measure, in amos. I haven't even found any research...

30 March 2024 1,577 2 View

What are the major difference between the Industry 4.0 and Industry 5.0?

In industry 4.0, major focus was on circular economy with automation.

30 January 2024 6,722 2 View

Why we convert Ag/AgCl potential and Hg/HgO potential into RHE in case of HER and OER?

In HER LSV plot is potential is measured into RHE so why we convert it from Ag/AgCl and what is the role of pH dependency

25 January 2024 9,059 0 View

Can we remove impurity peaks or stabilize the phase by recalibration?

I'm currently working on LLZO and I'm getting little impurity peaks in xrd. So is it possible that by recalcination we can remove those peaks?

12 January 2024 8,150 1 View

Scie list of mathematics during the years 2012, 2013, 2014?

I am in need of SCIE list of mathematical journals during 2012, 2013, 2014. If anyone has the list, please send. Also, the impact factor list during these years. I shall be thankful. With Sincere...

09 January 2024 3,288 0 View

How to plot Effective permittivity and permeability variation with respect to frequency for FSS unit by using HFSS Software ?

The sample graph is attached please anyone explain how to plot the attached graph in HFSS software.

03 December 2023 8,122 1 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How to assess the osmotic power of a substance?

Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...

06 August 2024 977 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View