Why crude fibre is present in protein isolates?

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How to compare two group with small sample size (n=3)?

I am working on some physiological traits of plant to which its expensive to raise the sample size beyond n=3. so kindly suggest some test which is applicable to test such group(2)

04 February 2021 461 11 View

How or from where can I get energy load profiles for the household, tertiary and industrial sectors specifically for EU27 countries?

Dear Researchers, I am looking for time series or a function to generate yearly, monthly or daily load profiles for the sectors such as household, tertiary and industrial. These load profile must...

11 January 2021 2,307 2 View

Why HIT PV modules have a lower degradation rate compared to multi-Si PV modules under humid environment?

Dear researchers, Greetings! Kindly provide me the explanation that why HIT PV modules have a lower degradation rate compared to multi-Si PV modules under humid environment? I have noticed that...

11 October 2020 6,410 2 View

What is the permissible limit of dye in industrial discharge?

what is the permissible limit of leather industrial dye? I will try to search the paper related to this topic but don't found.

20 September 2020 6,249 3 View

Is it necessary to construct calibration curve of drug in plasma for in-vivo pharmacokinetic study?

Is it necessary to construct the calibration curve of the drug in plasma for in-vivo pharmacokinetic study? Can we use simple calibration curve of drug constructed in solvent not in the plasma...

30 June 2020 6,949 5 View

Can anyone suggest a solidifying agent suitable for human consumption?

To make a food powder into porous solid.

15 June 2020 2,363 2 View

Which solar cell technology is suitable for 400nm-1660nm diffused radiation ?

which solar cell technology is more efficient for 400nm-1600nm diffused solar radiation and why?

27 April 2020 3,969 3 View

Is there any mathematical model to study the electric fringe field for parallel plates having equal and opposite potential?

Some Mathematical expressions will be helpful.

26 April 2020 8,000 2 View

What is the best measure of productivity of the faculty/researchers?

There are good number of measures are available to measure the research productivity of the faculty members/scientist like, 1.Scopus "h" Index of the author 2.Google Scholar "h' index of the...

20 February 2020 631 3 View

Experimental method for determination of C-H (homolytic) BDE in organic compounds?

Hello Everyone, We want to measure the C-H (homolytic) bond dissociation energy of some organic compounds. Any practically feasible tool in the common analytical laboratory that can give us the...

13 February 2020 7,588 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View