How to explain Epi Info Population formula?

Dear, I am using EpiInfo to analysis survey data. However there some issues that I still do not know how to figure out. What is the formula used to compute the Population survey. I found one n =...

10 November 2019 1,879 2 View

How does one compute IC50 value?

How to compute IC50 value of a solution automatically I performed experimentation, determining the percentage of bacteria growth in presence of different plant extracts and at different...

07 August 2014 4,708 3 View

Which statistical analyzes choose?

I want to perform test about anti-tumoral activity of triple dozen of plant extracts, with reference compounds. I have been told that before doing anything, I better choose the statistical test...

05 June 2014 9,399 2 View

What is the appropriate procedure to publish your first research article?

I have finished the manuscript of a research paper in French, and I would like to publish in English. But have not targeted a publishing group yet. which one could I choose? Do you have any...

05 June 2014 8,945 4 View

What does the R square coefficient mean and why is there different value when computing with Excel?

Computing the correlation between the concentration of plants extracts and the inhibitory percentage, I used excel software. When I make the Logarithm graph, in dialogue box, it is asked me if I...

04 May 2014 1,408 15 View

In an MTT viability test, what is the concentration of plant extracts that should be used?

I would like to study anti proliferative activity of plant extracts using the MTT test. I wonder which concentration of plant extracts I should used. What parameters should I take into account to...

03 April 2014 2,215 4 View

Is there a way to determine the cytotoxicty of a substance after assessing its viability effect?

I want to carry out the MTT test to show the viability effect of plant extract on cells lines. Is there a way to reveal the cytotoxic effects of these same extracts?

03 April 2014 5,167 3 View

What are the more relevant tests for assessing the anticancer activity of medicinal plants?

Anticancer tests methodology.

02 March 2014 2,431 3 View

On a computer running Ubuntu 13.04 OS, how to perform an LHD Fisher/HSD statistical analyses with R?

Software procedures: Make ANOVA 1 Analysis data with R software. Find out "P" and "F" with values during an ANOVA 1 Analysis of experimental data.

31 December 2013 4,846 2 View

Where could I apply for presenting a research result ?

I want to apply for presenting a communication in a conference.

31 December 2013 5,550 3 View

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02 March 2021 3,060 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

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28 February 2021 9,936 2 View