Is there a way to determine the cytotoxicty of a substance after assessing its viability effect?

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How to explain Epi Info Population formula?

Dear, I am using EpiInfo to analysis survey data. However there some issues that I still do not know how to figure out. What is the formula used to compute the Population survey. I found one n =...

10 November 2019 1,879 2 View

How does one compute IC50 value?

How to compute IC50 value of a solution automatically I performed experimentation, determining the percentage of bacteria growth in presence of different plant extracts and at different...

07 August 2014 4,708 3 View

Which statistical analyzes choose?

I want to perform test about anti-tumoral activity of triple dozen of plant extracts, with reference compounds. I have been told that before doing anything, I better choose the statistical test...

05 June 2014 9,399 2 View

What is the appropriate procedure to publish your first research article?

I have finished the manuscript of a research paper in French, and I would like to publish in English. But have not targeted a publishing group yet. which one could I choose? Do you have any...

05 June 2014 8,945 4 View

What does the R square coefficient mean and why is there different value when computing with Excel?

Computing the correlation between the concentration of plants extracts and the inhibitory percentage, I used excel software. When I make the Logarithm graph, in dialogue box, it is asked me if I...

04 May 2014 1,408 15 View

In an MTT viability test, what is the concentration of plant extracts that should be used?

I would like to study anti proliferative activity of plant extracts using the MTT test. I wonder which concentration of plant extracts I should used. What parameters should I take into account to...

03 April 2014 2,215 4 View

Why are there so many different methodologies for the MTT test?

I want to carry out the MTT Test, but in the literature, there is no standard test. The methodologies vary a lot from one another. Some people talk about shaking before optical reading, some...

03 April 2014 9,945 0 View

What are the more relevant tests for assessing the anticancer activity of medicinal plants?

Anticancer tests methodology.

02 March 2014 2,431 3 View

On a computer running Ubuntu 13.04 OS, how to perform an LHD Fisher/HSD statistical analyses with R?

Software procedures: Make ANOVA 1 Analysis data with R software. Find out "P" and "F" with values during an ANOVA 1 Analysis of experimental data.

31 December 2013 4,846 2 View

Where could I apply for presenting a research result ?

I want to apply for presenting a communication in a conference.

31 December 2013 5,550 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

How can plant phytochemistry be included in discussions about whole plant traits?

I am using the concept of plant functional traits (Cornellisen et al., 2003) in a paper. Considering descriptions for whole plant traits (life history; life form; growth form; plant height;...

24 February 2021 6,831 5 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View