Bradford calibration
Thanks alot sir.
Also sir i've read in net that peoples belives 'if the value of r 2 is greater than 0.95 then it is fine' but why so sir?
Which laptop should I buy (latest model) for working in computational physics? For performing molecular dynamics simulation, docking, DFT and modelling etc
07 May 2023 2,533 3 View
experts opinions are needed on this
19 December 2022 8,013 5 View
Hi users, I want to create an itp file for my ligand (ligand with ruthenium) complex, im using MCPB.Py for generating itp for my structure during the process i got the following error kindly...
22 November 2022 3,823 0 View
In general, the communication between the inter-cluster nodes has some linking issues due to the behavioral attributes of the sensor nodes. Hence the delay occurred to transmit the message or...
12 September 2022 2,263 0 View
in the case of two-photon and three-photon absorption, in most of the papers, the absorption cross-section values follow with absorption coeffcint. whether is there any exceptional case is there?...
20 June 2021 6,252 2 View
Hi, I am planning to test a drug that is known to reduce phosphorylation of RNA poll II at Ser2. My question is when I collect lysate for western do I need to do nuclear fractionation or is whole...
29 September 2020 1,501 4 View
Is it is possible to identify cone nuclei from rod nuclei in the outer nuclear layer as I need to know which cell is undergoing change. Thanks
17 June 2020 7,758 4 View
With the COVID-19 pandemic, the face mask is having its hour in the spotlight. Designers are making fashion masks; painters are creating one-of-a-kind mask masterpieces; companies are ‘masking’...
10 June 2020 8,160 18 View
Marketplace Simulations are impacting the lives of marketing students around the world. Simulations offer quality, experience-based learning opportunities that put students in the driver’s seat,...
07 June 2020 3,445 9 View
We often hear employers and business leaders lament the unfortunate gap between what students learn in college and what they are actually expected to know in order to be job-ready. This is...
04 June 2020 3,660 1 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View