What cut-off should we use when applying BUSCO to Escherichia coli (Bacteria) genome assemblies?

We would like to analyse thousands of E. coli genomes and identify orthologous genes. Before this we would like to filter the bad quality genomes by BUSCO (https://busco.ezlab.org/). What % of...

15 November 2020 5,152 3 View

Lyophilized Antibodies for Microfluidic Device?

I am using monoclonal lyophilized antibodies for a microfluidic chip which I would like to be stored at room temperature. What are the easiest methods to reactivate these antibodies for...

31 March 2018 8,941 2 View

What the best method for Research about Fruit Flies (Bactrocera spp)?

Entomology, Phytopathology, Integrated Pest Management

05 March 2017 6,708 14 View

How do i measure body size/image incongruence?

Hi. I am looking for a way to measure body size or body image incongruence. i would like to try and measure the differnece between actual and perceived body size. i was wondering if anyone knows...

11 December 2016 6,056 8 View

Are there any specific antibodies or markers for M phase of cell cycle?

07 December 2016 4,014 3 View

Can anyone recommend a good dissociation questionnaire, other than the DES?

i am looking for a good dissociation questionnaire, other than the DES. i would like something that had been validated and has been cited often.

10 November 2015 1,929 7 View

Why do dividing RPTECs show two different cluster morphologies?

We are currently culturing immortalized renal proximal tubules (RPTEC/TERT1) at passage 1. The cells are adherent, and so grow in clusters, but strangely, we observe two different types of...

24 June 2014 5,073 2 View

Does leaving (dilute) trypsin in with the cells during passage slow their growth?

I am growing hCMEC/d3 cells in Lonza EGM media with Bulletkit supplements. The passage protocol I use calls for just directly adding the resuspended cells, trypsin (.25% in EDTA) and all, to the...

03 March 2014 6,717 5 View

Apakah mahasiswa bisa dilibatkan dalam project ini?

saya mahasiswa tingkat akhir yang ingin mencari project penelitian untuk tugas akhir , saya adalah mahasiswa fisika saya tertarik dengan remote sensing pemetaan dan tomografi, mungkin jika...

01 January 1970 7,177 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

No mRNA increase in PCR after successful transfection in ARPE-19 cells ?

Hello, i am a bit curious about my results. I transfected the human cell Line ARPE-19 with a Plasmid containing a GFP tagged p53 (p53-GFP, Plasmid #11770). After 24h i checked under the...

18 February 2021 3,811 7 View

Which detection method is better for an ELISA-based assay: colorimetric or fluorometric?

Hello, I am planning to perform an ELISA-based assay to quantify the percentage of genomic methylation (5-methylcytosine) in human embryonic stem cells. I've been searching for some kits and I...

16 February 2021 8,389 3 View