I believe it has to do with protein size, or perhaps it may be due to the fact that introns (which are needed for interleukin production) are much more rare in bacterial genes than in eukaryotic cells.
I want to make sure that the freezing/thawing does not significantly affect the yield or quality of the extracted RNA. They would be frozen for at most a few months.
10 June 2021 2,038 3 View
I’ve been measuring the response of type 1aA diamonds to 405 nm laser and have observed several different transmission spectra. Some have significant fluorscsince while others barely. Just...
05 April 2021 5,207 4 View
I am not exactly sure how to describe this. I need to find out what the greatest height is between the perimeter of polygons I have and a point within the polygon that "stands up higher" than...
20 September 2020 6,576 7 View
The incubation is a 2-12 hour incubation at 37 C. I would like to use the bead bath because the usual incubator is not available. Would this be safe for the cells?
06 July 2020 3,567 0 View
The complete form of this adjuvant uses heat inactivated and dried mycobacteria. However, to rule out cross-reactivity with an antibody, I would like to know which proteins are left in the...
03 May 2020 1,527 2 View
I am working on a new publication trying to explain marine geomorphology of a reef system. I wanted to make a series of images showing how the geomorphology may have changed with changing sea...
16 July 2019 7,030 3 View
Anybody has any experience/pointers as to how best perform whole-cell on a DRG neuron and then lift it from the substrate to continue recording? We are plating the DRG cells in very low...
01 February 2019 1,013 3 View
I am trying to do a plasmid purification on my recombinant E.coli. strted from the midiprep kit by MN, than moved to the miniprep kit, by sigma, which we tried several times. all purification...
30 January 2019 7,591 9 View
Any recommendations for some good CRM case studies for an undergraduate course in Customer Value and Relationship Marketing? I'm looking for cases that are current (2016 or later), short (6-12...
18 January 2019 5,706 4 View
In my lab, mice infected with tuberculosis develop regional inflammation in the first stages of disease. Therefore, to avoid unrepresented sampling (too much or too little inflamation), we want to...
07 August 2018 3,120 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I have done an experiment using luminex technique, measuring 46 analytes. but my sample size is less (N=6, for case and control each). However, i have put up the experimental set in duplicates....
31 July 2024 3,665 3 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.
26 July 2024 7,387 0 View
I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...
25 July 2024 5,001 2 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View