What is the protein composition of complete Freund's adjuvant?

The complete form of this adjuvant uses heat inactivated and dried mycobacteria. However, to rule out cross-reactivity with an antibody, I would like to know which proteins are left in the...

03 May 2020 1,332 2 View

Do you have suggestions for programs to use to make a marine geomorphology reef cartoon image for publication?

I am working on a new publication trying to explain marine geomorphology of a reef system. I wanted to make a series of images showing how the geomorphology may have changed with changing sea...

16 July 2019 6,896 3 View

Lifting DRG neurons after whole-cell patch?

Anybody has any experience/pointers as to how best perform whole-cell on a DRG neuron and then lift it from the substrate to continue recording? We are plating the DRG cells in very low...

01 February 2019 931 3 View

Can anyone recommend an RNA extraction protocol for complete mouse lungs (>100 mg of weight) using silical-gel columns?

In my lab, mice infected with tuberculosis develop regional inflammation in the first stages of disease. Therefore, to avoid unrepresented sampling (too much or too little inflamation), we want to...

07 August 2018 3,011 2 View

Saliva DNA Extraction Incubation Time?

Hello, I am currently working through a set of saliva samples for DNA extraction. We have been following a protocol that begins by combining the sample with 60 ul of Proteinase K and 300 ul of...

21 June 2018 4,522 2 View

What is the time limit to place a whole brain sample in liquid nitrogen after cervical dislocation has taken place, for appropriate RNA extraction?

Besides obtaining the brain for RNA extraction, I need to obtain other organs in the mouse, such as the spleen, to measure neurotransmitters using HPLC (neurotransmitters degrade very rapidly!)....

11 December 2017 6,762 6 View

Do bioerosional grazing urchin and parrotfish favor endolithic or epilithic algae? When eating epilithic algae, do they still erode substrate?

06 August 2017 6,067 4 View

Urgent: does this analysis of a repeated measure manova make sense?

background: 100 students were given an unrelated text and a related text each. they then had to answer questions on each text, say how interested they were in each text, and then evaluate how much...

30 May 2016 6,467 3 View

What is the optimal lysis buffer to use for pharmacologic specific protein binding assay?

Starting material is immune cells. The receptor of interest is located in mitochondrial membrane. We are using radiolabeled ligand for this protein so we want to avoid or at least minimize protein...

13 May 2014 6,956 1 View

What is an effective chemical etchant to remove surface Te from a ZnTe film?

My lab is working with a ZnTe MBE film grown on GaSb. We etch of the GaSb substrate, but find (via Raman) Te on the remaining ZnTe-film surface. It is difficult to remove. Does anyone know what...

04 June 2013 8,434 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

How to accurately measure cytokines levels for my university research proposal?

Hello, i am writing a theoretical research proposal for one of my university assignments regarding Covid-19. The brief is pretty relaxed and i have chosen to conduct a study on how the...

11 February 2021 908 5 View