The incubation is a 2-12 hour incubation at 37 C. I would like to use the bead bath because the usual incubator is not available. Would this be safe for the cells?
Hello, We perfom Tokuyasu method (ultrathin cryo sectionning) very often to make immunogold experiments. This time, we need to make a PLT technique with K4M or LR white resin. Our results were...
14 February 2021 4,959 1 View
Hello, We tried to do CLEM experiment on sapphire disk including cryo fixation (Leica ICE) and then cryo substitution using LR white resin. Once the resin is polymerized, we can't remove the...
13 January 2021 5,223 3 View
We would like to control the honeybees' ID at the entrance of a flight tunnel. We would like to detect a honeybee in a cube of 10x10x10 cm3 in front of the tunnel door while flying, and thus...
17 December 2020 3,464 2 View
What are the competencies to put forward in Higher Education to prepare students to evolve in a world where IA is ubiquituous?
15 December 2020 6,350 4 View
There are many tools to build a PLSR model to predict a response variable Y based on a multivariate predictive variable X (reflectance spectra for example). My question is the following: once we...
06 December 2020 3,096 11 View
Hello, As you must know, it is now sometimes possible to have several co-first authors in the co-authorship of a scientific publication, when two persons led the paper and had equal contribution...
21 September 2020 2,995 5 View
I'm reviewing a lot of papers where the authors take a 3-D autonomous chaotic system (think Lorenz) and add a fourth variable bidirectionally coupled to the other three and then report its unusual...
04 May 2020 3,785 33 View
The complete form of this adjuvant uses heat inactivated and dried mycobacteria. However, to rule out cross-reactivity with an antibody, I would like to know which proteins are left in the...
03 May 2020 1,332 2 View
Hello everyone The UMI3D Consortium's working group dedicated to embodiment is currently looking for a device agnostic way to manage navigation in Collaborative Virtual Environment (CVE). Are you...
08 April 2020 4,951 3 View
Hi everyone. I am in the process of making RDAs between faunistic and environmental tables. What I don't understand is that depending on the order in which I place my environmental variables...
29 March 2020 2,179 5 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...
01 March 2021 6,999 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...
27 February 2021 8,128 1 View
I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...
23 February 2021 7,842 1 View
I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...
23 February 2021 9,933 1 View
New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...
21 February 2021 3,278 3 View
Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...
21 February 2021 7,397 1 View
I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...
21 February 2021 6,260 3 View