The incubation is a 2-12 hour incubation at 37 C. I would like to use the bead bath because the usual incubator is not available. Would this be safe for the cells?
Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...
23 July 2024 6,314 3 View
Hello everyone. During my analyses on LC MSMS, the area ratio (Standard Area/Isotopically labelled Standard Area) increases with time for the quality control point. I'm having trouble explaining...
08 July 2024 3,150 2 View
Do you know systematic reviews and/or meta-analyses that have chosen to exclude articles from (or suspected to be) predatory journals/publishers in their work ?
24 March 2024 4,941 3 View
Is it possible to keep them in -80 freezer or should we keep them in liquid nitrogen?
17 July 2023 2,846 1 View
We're looking for a complete system for producing anodized aluminum oxide (AAO) substrates from self-supported aluminum foil?
12 June 2023 3,526 2 View
Dear members, As you know, the identification of urinary tract infection by culture is crucial for the diagnosis and treatment. However, traditional methods of culture identification can be...
04 May 2023 7,178 3 View
Hi, I need humanized mouse models for flavivirus infection but I do not have the skills to produce them. Do you know of a company that can do it. Thank you very much
20 February 2023 3,058 0 View
Hello everyone, I'm currently doing some DTA analysis on two phases of the same ceramic material, one is fully crystalline and the other fully amorphous. When I work with the crystalline one I...
03 July 2022 8,061 3 View
I would like to perform some microscopy experiments on yeast zygots. Unfortunetly zygots are clumping together which is not ideal condition for my experiment. I tried to add 0.1% of Tween80 with...
01 March 2022 8,606 2 View
Hi, I'm a fish biologist and I'm interested in assessing the uncertainty around the L50, which is the (sex-specific) length (L) at which you expect 1 fish out of 2 (50%) to exhibit developed...
21 November 2021 173 11 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it
06 August 2024 947 1 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?
15 July 2024 1,598 3 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...
10 July 2024 6,830 1 View
Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...
10 July 2024 4,918 2 View