Leon Islas-Weinstein

3 Questions 4 Answers 0 Followers

Questions related from Leon Islas-Weinstein

Can anyone recommend an RNA extraction protocol for complete mouse lungs (>100 mg of weight) using silical-gel columns?

In my lab, mice infected with tuberculosis develop regional inflammation in the first stages of disease. Therefore, to avoid unrepresented sampling (too much or too little inflamation), we want to...

08 August 2018 3,070 2 View

What is the time limit to place a whole brain sample in liquid nitrogen after cervical dislocation has taken place, for appropriate RNA extraction?

Besides obtaining the brain for RNA extraction, I need to obtain other organs in the mouse, such as the spleen, to measure neurotransmitters using HPLC (neurotransmitters degrade very rapidly!)....

12 December 2017 6,818 6 View

Why are bacteria incapable of naturally producing interleukins?

I believe it has to do with protein size, or perhaps it may be due to the fact that introns (which are needed for interleukin production) are much more rare in bacterial genes than in eukaryotic...

06 June 2017 8,790 0 View