Best protocol for immunogold using araldite resin ?

Hello, We perfom Tokuyasu method (ultrathin cryo sectionning) very often to make immunogold experiments. This time, we need to make a PLT technique with K4M or LR white resin. Our results were...

14 February 2021 4,959 1 View

How to remove a sapphire disk from LR white resin embedding ?

Hello, We tried to do CLEM experiment on sapphire disk including cryo fixation (Leica ICE) and then cryo substitution using LR white resin. Once the resin is polymerized, we can't remove the...

13 January 2021 5,223 3 View

What do you suggest me as a low-cost electronic monitoring of honeybees using 3-5mg RFID tags?

We would like to control the honeybees' ID at the entrance of a flight tunnel. We would like to detect a honeybee in a cube of 10x10x10 cm3 in front of the tunnel door while flying, and thus...

17 December 2020 3,464 2 View

What are the competencies to put forward in Higher Education to prepare students to evolve in a world where IA is ubiquituous?

What are the competencies to put forward in Higher Education to prepare students to evolve in a world where IA is ubiquituous?

15 December 2020 6,350 4 View

Is it possible to invert a PLSR model, i-e to predict X based on Y?

There are many tools to build a PLSR model to predict a response variable Y based on a multivariate predictive variable X (reflectance spectra for example). My question is the following: once we...

06 December 2020 3,096 11 View

Have you ever included two last authors in the co-authorship of a publication?

Hello, As you must know, it is now sometimes possible to have several co-first authors in the co-authorship of a scientific publication, when two persons led the paper and had equal contribution...

21 September 2020 2,995 5 View

How does one find constants of the motion for a chaotic flow?

I'm reviewing a lot of papers where the authors take a 3-D autonomous chaotic system (think Lorenz) and add a fourth variable bidirectionally coupled to the other three and then report its unusual...

04 May 2020 3,785 33 View

What is the protein composition of complete Freund's adjuvant?

The complete form of this adjuvant uses heat inactivated and dried mycobacteria. However, to rule out cross-reactivity with an antibody, I would like to know which proteins are left in the...

03 May 2020 1,332 2 View

Device abstraction for navigation in VR ?

Hello everyone The UMI3D Consortium's working group dedicated to embodiment is currently looking for a device agnostic way to manage navigation in Collaborative Virtual Environment (CVE). Are you...

08 April 2020 4,951 3 View

Redundancy analysis (RDA) order variables change result, why?

Hi everyone. I am in the process of making RDAs between faunistic and environmental tables. What I don't understand is that depending on the order in which I place my environmental variables...

29 March 2020 2,179 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View