Why appear two bands one of my expected protein and another around 17kDa?

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Christian Q. Scheckhuber

Hello Aldo,

the smaller protein could be protease-degraded protein. What kind of protease inhibitor-mix do you use?

Regards

Debaprasad Koner

Dear Montoya,

Kindly go through the link below. You might get some ideas.

https://www.researchgate.net/post/Double_band_for_a_single_protein_on_western_blot

With regards,

Koner