i have two bands in my western blot, i used histag antibody and HRP as secondary antibodie, the samples are recombinant proteins in ecoli, someone toldme that extra band is an heterogeneus protein of e.col but i dont find any article about it, the MW of my target protein is 28KDa, the MW of the other band is around 17KDa
Christian Q. Scheckhuber
Hello Aldo,
the smaller protein could be protease-degraded protein. What kind of protease inhibitor-mix do you use?
Regards
1 votes
0 thanks
Debaprasad Koner
Dear Montoya,
Kindly go through the link below. You might get some ideas.
https://www.researchgate.net/post/Double_band_for_a_single_protein_on_western_blot
With regards,
Koner
2 votes
0 thanks
- Badges
- Science method
More Aldo Adrián Pérez Montoya's questions See All
Similar questions and discussions