Debaprasad Koner

12 Questions 32 Answers 0 Followers

Questions related from Debaprasad Koner

Can I use Mitored dye for microscopic studies in Isolated mitochondria?

I have isolated mitochondria from tissue. I would like to check them by fluorescence imaging. Can I use Mitored dye for these isolated mitochondria?

26 September 2021 5,326 2 View

How to check tissue specific distribution of mRNA using Real-time PCR?

I just want to check the expression of a particular mRNA throughout the different tissues (Liver, Kidney, Brain, Muscle, Gills, Intestine, Ovary, Skin, Spleen etc.) in a control/untreated fish. If...

28 February 2018 2,291 6 View

Why only BSA and Skimmed milk proteins are used for blocking in Western blotting?

Why antibodies (primary and secondary) do not cross react with these protein? What chemistry is there to choose these proteins as blocking reagent?

09 January 2018 2,128 6 View

Have anyone used Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit for H2O2 quantification from cell using Qubit?

Dear Researchers, I am using Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit (Thermo Fischer) for H2O2 quantification from cell. There is an option for Qubit but, there is no clear protocol for...

03 November 2016 5,878 2 View

What concentration of Aminoguanidine Bicarbonate Salt should I use in cell culture to inhibit inducible nitric oxide synthase (iNOS)?

I want to perform an inhibition study of iNOS activity in primary hepatocyte by using aminoguanidine. What concentration of aminoguanidine in cell culture should I choose? Thanks in advance.

04 July 2016 9,665 6 View

Is there any technical difference to express enzyme activity between U/gm wet wt and U/mg of protein?

In some article, the enzyme activity has shown in terms of gm wet wt but, I used to measure in terms of mg of protein. Is there any difference (technical, bio-chemical) between these?

10 March 2016 7,780 7 View

What is the best method to assay MDA?

I am getting different concentration values for the same sample. For liver tissue, at first I got 35 µM concentration. But, after one day the concentration value has come 13.5 µM.To assay MDA from...

02 March 2016 3,781 8 View

How to assay GSH and GSSG?

Kindly help with the protocol of end point assay for GSH and GSSG. Thanks in advance.

11 February 2016 7,976 11 View

What should be the unit of Nitric Oxide in tissue?

I have estimated Nitric Oxide from tissue homogenate by using griess reagent. From the standard curve, I got a concentration about 12 µM. I have used 100 µl of tissue homogenate. The protein...

29 January 2016 4,745 4 View

How to calculate SOD activity using nbt and riboflavin?

How should I calculate SOD activity? How can I calculate the percentage of inhibition? I am using spectrophotometric method. Thanks in advance.

29 January 2016 7,808 9 View

Should I use DTT (Dithiothreitol) or any reducing agent in homogenizing buffer for enzyme assay?

I want to assay the enzyme activity of different antioxidant enzymes. Should I use any reducing agent in homogenizing buffer? I have gone through some protocols for assay and the reducing agents...

25 January 2016 8,398 20 View

What is the significance of molar extinction coefficient in enzyme kinetics assay?

What does it actually imply?

17 January 2016 2,707 3 View