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Questions related from Debaprasad Koner
I have isolated mitochondria from tissue. I would like to check them by fluorescence imaging. Can I use Mitored dye for these isolated mitochondria?
26 September 2021 5,337 2 View
I am searching for the protocol of Chromatophores isolation and culture from fish skin. Can anyone kindly guide me regarding the experiment? Thanks in advance.
18 November 2019 6,752 5 View
I just want to check the expression of a particular mRNA throughout the different tissues (Liver, Kidney, Brain, Muscle, Gills, Intestine, Ovary, Skin, Spleen etc.) in a control/untreated fish. If...
28 February 2018 2,315 6 View
Why antibodies (primary and secondary) do not cross react with these protein? What chemistry is there to choose these proteins as blocking reagent?
09 January 2018 2,153 6 View
I want to perform an inhibition study of GR in fish primary hepatocytes. Is there any Glutathione reductase specific inhibitor which can inhibit only GR not other disulfide oxidoreductase? Thanks...
14 February 2017 3,308 4 View
Dear Researchers, I am using Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit (Thermo Fischer) for H2O2 quantification from cell. There is an option for Qubit but, there is no clear protocol for...
03 November 2016 5,907 2 View
I have measured H2O2 in primary hepatocytes of fish. How should I express the concentration? in terms of mg of protein or in terms of number of cells?
12 October 2016 4,393 3 View
I want to perform an inhibition study of iNOS activity in primary hepatocyte by using aminoguanidine. What concentration of aminoguanidine in cell culture should I choose? Thanks in advance.
04 July 2016 9,677 6 View
I want to quantify NO using griess reagent from tissue. I have made 10% tissue homogenate and centrifuged at 12,000 rcf for 10 mins. I have taken the supernatant for NO estimation. Should I...
01 June 2016 1,133 3 View
In some article, the enzyme activity has shown in terms of gm wet wt but, I used to measure in terms of mg of protein. Is there any difference (technical, bio-chemical) between these?
10 March 2016 7,788 7 View
I am getting different concentration values for the same sample. For liver tissue, at first I got 35 µM concentration. But, after one day the concentration value has come 13.5 µM.To assay MDA from...
02 March 2016 3,794 8 View
Kindly help with the protocol of end point assay for GSH and GSSG. Thanks in advance.
11 February 2016 7,987 11 View
I have estimated Nitric Oxide from tissue homogenate by using griess reagent. From the standard curve, I got a concentration about 12 µM. I have used 100 µl of tissue homogenate. The protein...
29 January 2016 4,772 4 View
How should I calculate SOD activity? How can I calculate the percentage of inhibition? I am using spectrophotometric method. Thanks in advance.
29 January 2016 7,818 9 View
I want to assay the enzyme activity of different antioxidant enzymes. Should I use any reducing agent in homogenizing buffer? I have gone through some protocols for assay and the reducing agents...
25 January 2016 8,426 20 View
What does it actually imply?
17 January 2016 2,731 3 View
Dear experts, I am a bit confused with the expression pattern of antioxidant enzymes i.e CAT, SOD and GST. I am checking oxidative damage potentiality of certain nanoparticle on primary...
12 January 2016 4,629 12 View
To assay the GST activity from liver lysate, I have used 0.5 mM GSH, 0.5 mM CDNB and 10 µl of tissue supernatant. I have assayed with the increase in the absorbance at 340 nm. I have set blank...
23 December 2015 7,826 15 View
Catalase is tetrameric protein with a heme group in active site. As EDTA is strong metal chelating agent, there is a probability to affect the activity of the enzyme. In many commercial assay...
01 January 1970 3,534 4 View
