Hi Michael,

Since these cells metastasised to brain, could this be possible for you to see CD133 etc expression, then may be you could che 

May be could check stem cell marker because of distant localization and drug efflux activity by Flow, will be easy for you to characterise further.

Yes,

the problem here is mainly time - we are (as mentioned) a colorectal cancer lab with the "problem" of having to characterize at the moment more then 30 patient-derived CRC cell lines.

I will be happy to send the lung cancer cells around for further characterization - of course, after an MTA has been signed by the administration etc.

And we have some data (mostly on drug resistance, growth characterisitcs ... , but neither time nor sometimes the exact knowledge what to do best.

Stem cell markers may be of interest for some people - but at the moment the trend is just turning in the cancer field - and I think this is a good development since these properties may sometimes change pretty fast in cancer cells ;-)

Thanks again and best regards

Michael

I am working on lung cancer. How can I help you??

Please have a look in the answer above - I am looking for some lab who wants to cooperate with the goal to finally publish the cell line characterization. Positions will depend on the contribution :-)

Michael, So out of 30 CRC, how many of them metastasised to brain? 

We have one established from a brain met. But this is of course dependent on how many mets are collected and cannot be interpreted as a statistic of significant value :-)

Hi Michael

We are working on lung cancer and feel very interested in these cell lines. Just want to know more about these lines. You did lung cancer PDX and the subcutaneous transplanted tumors are able to  metastasize to brain, right? What's the percentage for this brain metastasis? Thanks!  

It is the same as for colon cancer cel lines….. In the case of lung carcinoma it is important to know the  final histological diagnosis (derived tumors) than there is a panel of mAbs that can be used to characterize the derived cell lines, depending if the originary tumors are SCLC, NSCLC, neuroendocrine tumors etc…. (i.e. CK7, p63, synaptofisina, cromogranina, and so on……)

Hi Hongbin,

no, the cell lines have been established from metastases of primarily lung carcinomas into human brain. And we did not yet perform any xenotransplantation with any of this material.

And Armando,

thanks for the suggestion - but as I mentioned before, I am looking for a lab which is a little more specialized towards lung-Ca as we are. It begins with the antibodies that you mention - I have currently no funding for an extra panel of antibodies for lung Ca.

Hi Michael,

Do you know the EGFR mutation status for these cells? Also, the cell subtype.....e.g. if it is adenocarcinoma or squamous etc......

No. :-(

Dear Michael

My lab is focussed on lung cancer.  I would be interested in talking to you further about this.  http://www.rob.ox.ac.uk/research/Andy%20Ryan

Dear Michael,

actually we are running a study focusing on lung primary tumor and brain metastases (at NCT-Heidelberg).

We already characterized lung cancer cell lines (see Gottschling et al. 2012, Lung Cancer).  Your cell lines might probably add value  to the project. We would be happy to get into contact with you and to exchange further details.

Best regards

Thomas

Dear Ryan, dear Thomas,

thank you for this feedback. I will contact you!

Michael

Dear Michael,

we can characterize your cells for miR-126 levels, which is downregulated in lung cancer and miR-126 restoration suppress tumor progression

Dear Marco,

which biomaterial would you need (or better what would you prefer?) to measure miR-126?

Hi Michael,

I can measure miR-126 on cells, colture medium, biopsies, serum