Madiha Kanwal

5 Questions 33 Answers 0 Followers

Questions related from Madiha Kanwal

Is there any way by which I can download Chinese population specific Reference gene sequence?

I want to compare my sequencing results with the reference gene sequence by Chinese population. Is there any database available online that can help me in downloading gene sequence? I have already...

04 April 2016 5,469 5 View

What is the best PCR Kit for EML4-ALK gene fusion?

I am trying to find out about the EML4-ALK gene fusion in NSCLC. I have difficulty in optimizing PCR. I wonder is there any PCR kit available which had been used for EML4-ALK gene fusion in...

07 July 2014 9,946 1 View

Why is my real-time PCR giving multiple peaks?

I am trying to check gene expression in a few cell lines, but the problem is that every time the results of Real-time PCR gives error 1 and 2, which means that in my sample cDNA concentration is...

05 May 2014 7,621 8 View

How do you get rid of a poor amplification problem?

I am trying to find gene fusion in cancer cell lines. I am using cDNA in conventional PCR. Forward and reverse primers belong to 2 different genes so I don't know the product size. I found a gene...

04 April 2014 1,902 14 View

How can I confirm gene fusion/mutation?

After we find a gene fusion by performing PCR and later confirm it with sequencing, how do we further confirm the clinical role of that gene fusion in cancer? What should be the methods to follow...

04 April 2014 1,093 2 View