Since we are a colorectal cancer lab, we would be happy to cooperate with a gastric cancer lab to characterize these two novel cell lines.
Hello Dr. Linnebacher, would you care to elaborate on the terms of cooperation? We have the pathology and also the possibility of electron microscopy.
Of course,
since I am mostly interested in getting our cell lines used, I would like to characterize them as good as possible or at least as good to allow for an interesting publication. So, I am offering co-authorships in exchange towards data - the normal scientific deal ;-)
Pathology would be interesting - we have the "standard" analysis of the primary tumors coming from our clinical pathology. In addition, we can perform FACS and most of the QC needed for cell lines: exclusion of mycoplasma, virus contamination, verification of human origin and of epithelial character. We typically do also test the duplication times, tumorigenicity in nude mice and therapy-responses towards standard drugs used in the clinics.
So, we would need somebody who wants to add and of course if the contribution is substantial enough, I have no problems with offering a first or last position.
I think this helps.
Best Michael
All right Dr.Linebacher, I'll consult with my fellows from the pathology lab ( unfortunately they will return on 9 .08) so there will be an justified delay, but hopefully the answer on our side is Y; my mail is [email protected]
Best regards
It should be not difficult to characterize these cell lines if they grow efficiently in vitro.
You can use an immunocyto/histochemical approach with three mAbs directed to antigens that are generally expressed in gastric cancer (citokeratin 20, CDX2, CEA)……
Hi, this could be interesting for us, we are doing transcriptome (RNAseq) and genomic mutational analysis and likely could add these two lines
Please contact me at my Columbia email if you would like to further discuss
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