Does re-stimulation of T cells with PMA and Ionomycine before staining for intracellular cytokines for flow cytometry affect reliability of the assay?

I understand that to be able to measure intracellular cytokines there is a need to restimulate with PMA and Ionomycin but I don't understand how the results will be reliable after that. PMA and...

18 January 2021 7,240 7 View

In meta-analysis of binary outcomes, what should we do when the occurrence of outcome is zero in both groups?

In conducting meta-analysis of binary outcomes (in RCTs), in case the risk ratio has not been reported, what should we do with the studies that the number of outcomes occurrence is zero in both...

09 January 2021 2,802 5 View

How to calculate ratio of means and ratio of geometric means?

In conducting a meta-analysis, sometimes, some studies have reported geometric mean, while others have reported arithmetic mean. One solution for pooling these studies is calculating the ratio of...

11 December 2020 4,663 3 View

How To Find Electrical Nanodipole and Magnetic Loop Dimensions At Optical Wavelength?

I would be grateful if you could suggest a paper or book which provides an equation to determine the dimensions (length) of an electrical nanodipole and magnetic loop antenna at desired optical...

17 October 2020 2,421 4 View

What effect do epidemics have on cerebral physiology?

How trauma and fear of infection can affect brain functions and structures?

10 October 2020 6,642 1 View

Can anyone advise me in selecting a good pair of universal primer/primers for identification of lactic acid bacteria (probiotics)?

when I blast the existed primers specially 16s rDNAs , I cant get good results.

21 September 2020 1,584 4 View

Could anyone help me searching for protocols in fiber analyses ?

Hello, I am looking for protcoles to evaluate the following components: TDF : total dietary fiber, SDF : soluble dietary fiber, IDF : insoluble dietary fiber et TDN : total digestive nutrients.

06 September 2020 371 2 View

Any journal which has no publication fess for pops removal or water treatment through adsorption?

I am looking for a reputable journal for publication of research article of MS thesis. Kindly suggest me some journals related to water treatment through adsorption which is free of cost.

13 July 2020 8,259 6 View

How much water we should add to paint if we use a based water binder (resin) or there is no need for adding water with such binder?

I want to know if the water included in resin is enough as a solvent or that water is not counted and I should add water as solvent to paint?

21 June 2020 322 6 View

In your opinion, do I choose a certain disorder to study its relationship with culture, or better not to define the disorder?

Dear colleagues Who can help me find studies or references that study the relationship of culture to mental disorders. I would be very grateful for your help. :)

26 April 2020 3,003 36 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

How to improve and implement WBAN MAC and Radio modules under Castalia 3.2 ?

Hello researchers, Hello Castalia3.2 researchers I am a beginner with Castalia, I work on optimization of RFID systems used in wireless body networks. I simulated a WBAN with 10 nodes and one...

28 February 2021 400 1 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View