Hello!
I have a problem with my purification step. I have a single strand dna (after a digestion using Lambda Exonuclease) that I need to purify before injecting it as a dna donnor in C.elegans worms for a CRISPR experiment.
So, I do my PCR, my digestion, I verify on gel that I have a single strand dna. Then, after the purification step using AMpure beads, I verifiy again on gel before injecting and that's when I find back my double strand dna (on the gel, I see a band for the double strand which becomes the majority of my sample and a small fine line for the single strand that I had before the purification step)
So, if anyone had encountered this kind of problem with this purification step and could help me with some tips on how to improve my results I would be grateful.
Thank you!
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